Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,489,130	A→G	V26A (GTG→GCG)	glpR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,489,130	0	A	G	100.0%	109.3 / NA	31	V26A (GTG→GCG)	glpR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/17);  total (14/17)

CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTG  >  REL606/3489055‑3489200                                                                            |                                                                       gtgaGGATCAGGGGTTGCTCCGACAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTcca                                                                        <  1:568588/73‑1 (MQ=255)    aGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTccgcca                                                                     >  2:770502/1‑76 (MQ=255)      gATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGc                                                                   >  1:60056/1‑76 (MQ=255)       aTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGGGCTCCGCCCGct                                                                  >  1:357561/1‑76 (MQ=255)         cAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGcgcc                                                                >  2:283532/1‑76 (MQ=255)          aGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGctccn                                                               >  1:683107/1‑75 (MQ=255)              tttGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGt                                                           >  2:106735/1‑76 (MQ=255)                   tCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGa                                                      >  2:521378/1‑76 (MQ=255)                      gCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACAt                                                   <  1:242638/76‑1 (MQ=255)                       ccAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATa                                                  <  1:299008/76‑1 (MQ=255)                       ccAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATa                                                  <  2:574367/76‑1 (MQ=255)                        cAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATaa                                                 <  1:399977/76‑1 (MQ=255)                           cTCATTGAGGTCGCGGAGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAAccc                                              <  2:329155/76‑1 (MQ=255)                            tCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCct                                             >  2:295303/1‑76 (MQ=255)                                tGAGGTCGCGGCGAATAGTCTGCNGTCTGACNGNGAAGTGCTCCGTCGGCTCCTCGGTGCAGACGTAACCctgctg                                         >  1:730284/1‑76 (MQ=255)                                tGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCctgctg                                         <  1:362230/76‑1 (MQ=255)                                 gAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGt                                        <  2:488776/76‑1 (MQ=255)                                      cgcgGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGGACTGACATAACCCCGCTGTTTAAc                                   >  2:262326/1‑76 (MQ=255)                                        gtgcgAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCa                                 <  1:713523/74‑1 (MQ=255)                                         ggcgAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAg                                >  1:225140/1‑76 (MQ=255)                                            gAATAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                             >  2:419512/1‑76 (MQ=255)                                             aaTAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                            <  1:253826/76‑1 (MQ=255)                                             aaTAGTCGGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCTTGCTGTTTAACCAGTTCg                            <  2:430465/76‑1 (MQ=255)                                              aTAGTCTGCGGGCTGACGGGGAAGGGCTCCGCCAGCTCCTCGGGACTGACATAAACCTGCCGTTTAACCAGATCGa                           >  2:194951/1‑76 (MQ=255)                                              aTAGTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTAGCGACATAACCCTGCTGTTTAACCAGTTCGa                           <  1:770506/76‑1 (MQ=255)                                               tggTCTGCGGGCTGACGGAGAANTGATCCGCCANCTCCTCGGNACTGACATAAGCCTGCTGGTTANCCAGTTCGat                          <  2:684962/74‑1 (MQ=255)                                                 gTCTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                        <  1:391760/76‑1 (MQ=255)                                                   cTGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                      >  2:40828/1‑76 (MQ=255)                                                    tGCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                     >  2:300016/1‑76 (MQ=255)                                                     gCGGGCTGACGGAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                    >  2:79162/1‑76 (MQ=255)                                                               ggAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt          >  2:152944/1‑76 (MQ=255)                                                               ggAGAAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTGAACCAGTTCGATAATACCGTTGTGACGt          >  2:708432/1‑76 (MQ=255)                                                                 agaAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg        <  2:204672/76‑1 (MQ=255)                                                                 agaAGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg        <  1:289802/76‑1 (MQ=255)                                                                   aaGTGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg      <  2:579529/76‑1 (MQ=255)                                                                      tGCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt   >  2:387407/1‑76 (MQ=255)                                                                       gCTCCGCCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg  <  2:183819/76‑1 (MQ=255)                                                                            |                                                                       CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTG  >  REL606/3489055‑3489200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑