| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 83.3% | 93.5 / 16.4 | 30 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base . (21/4); total (22/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131385‑2131531 | atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:475666/76‑1 (MQ=255)atgaTTTGTTTTGAAATGTCGANANAATGCTCACANAAACGACAATATGANAATTAATAGTGGAATAGAAGGCaat < 2:24603/76‑2 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:163041/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:616681/76‑1 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:251314/1‑76 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:959669/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:817261/1‑75 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:489260/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:674456/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:652281/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:394724/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGNAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:1212035/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAAAGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:958044/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:249484/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:861173/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCAGAGGGa > 1:1398904/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 2:1244377/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:1259888/1‑76 (MQ=255) gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGca > 2:1268172/1‑76 (MQ=255) gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGca < 2:222634/76‑1 (MQ=255) tCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCCCCCGGAGGGAAGCCGcaac < 2:127629/76‑1 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaaca > 2:297808/1‑76 (MQ=255) aaaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa < 2:110876/75‑1 (MQ=255) aaaaCAACAATATGAAAATAAAAAGAGAAATGAAAGG‑AAAAAAAACCGGCCTCGCGGAGGGAAGCCGCAACAAAGt < 2:1123564/76‑1 (MQ=255) aaCGACAATATGAAAATTAATAGAGGAATGAANGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAANTgc > 2:1323704/1‑76 (MQ=255) aaCGAAAATATAAAAATTAATAGAGGAATAAAAGGAAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg < 2:862597/76‑1 (MQ=255) atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 1:1051876/1‑76 (MQ=255) taanaTTAGTAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGNAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:1333534/75‑1 (MQ=255) aaaaaTAAAAAGAGGAATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:659119/75‑1 (MQ=255) aaaaaGTAGTAAAGGAATAAAAAG‑AAAAAAACGCGGCCTCCCGGGGGAAAGCAGCAACAAAGTGCGCGTGGTTTTa < 2:659131/75‑1 (MQ=255) aaaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:4510/76‑1 (MQ=255) cagttAAAAGAGANATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATg < 1:183104/73‑1 (MQ=255) aaaTAAAAAGAGAAAAAAAAGAAAAAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 1:819562/76‑1 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 1:716690/1‑76 (MQ=255) tAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:458602/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:385959/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:804629/1‑76 (MQ=255) agGAATGAAAGG‑NAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 1:13044/1‑76 (MQ=255) gAAGGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 2:716690/76‑1 (MQ=39) aTGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:291765/76‑1 (MQ=255) tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:561079/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGCGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:882670/1‑76 (MQ=38) aaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:591037/1‑76 (MQ=255) aaaaaaaaaacCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 1:1092620/74‑1 (MQ=38) | ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131385‑2131531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |