Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	100.0%	152.9 / NA	39	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (20/19);  total (20/19)

TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695                                                                            |                                                                        tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑t                                                                       <  2:1123347/76‑1 (MQ=255)  aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tt                                                                      <  2:866304/76‑1 (MQ=255)   gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttt                                                                     >  1:955298/1‑76 (MQ=255)     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttt                                                                   <  1:1024405/76‑1 (MQ=255)     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttt                                                                   <  2:752360/76‑1 (MQ=255)      ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttt                                                                  >  2:77226/1‑76 (MQ=255)       tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttt                                                                 <  2:1228832/76‑1 (MQ=255)          tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttttca                                                              >  2:817420/1‑73 (MQ=255)              cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGt                                                           <  1:849071/76‑1 (MQ=255)               aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTg                                                          >  1:1153747/1‑76 (MQ=255)               aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTg                                                          >  2:929877/1‑76 (MQ=255)               aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTg                                                          >  2:929869/1‑76 (MQ=255)                tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGa                                                         >  1:1120958/1‑76 (MQ=255)                  cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGaaa                                                       >  2:327436/1‑76 (MQ=255)                     gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATgg                                                    <  2:357176/76‑1 (MQ=255)                      tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGa                                                   >  2:1250277/1‑76 (MQ=255)                        cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACa                                                 <  1:804198/76‑1 (MQ=255)                        cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACa                                                 <  1:702035/76‑1 (MQ=255)                        cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACa                                                 >  1:1006292/1‑76 (MQ=255)                         tGACTGTAATTTGAGAACTAATTTAAACCTCTTCTCCTTAAAAAAACTACAT‑TTTTTTTCATCGTAAAATGGACat                                                <  2:391685/76‑2 (MQ=255)                          gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACaga                                               >  2:868032/1‑76 (MQ=255)                          gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACaga                                               >  2:868019/1‑76 (MQ=255)                              gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGAt                                           <  1:724261/76‑1 (MQ=255)                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATa                                          <  2:702448/76‑1 (MQ=255)                                aaTTTGAGAACGAATTTAACCCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaa                                         >  2:1022524/1‑76 (MQ=255)                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaa                                         >  1:336791/1‑76 (MQ=255)                                     gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGt                                    >  1:957149/1‑76 (MQ=255)                                      agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt                                   <  2:379527/76‑1 (MQ=255)                                         aCGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                                <  1:492760/76‑1 (MQ=255)                                         aCGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                                <  1:492759/76‑1 (MQ=255)                                           gAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa                              <  2:272612/76‑1 (MQ=255)                                                 aaaCCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        >  2:129323/1‑76 (MQ=255)                                                  aaCCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  1:1097832/76‑1 (MQ=255)                                                      gCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc                   >  1:1162808/1‑76 (MQ=255)                                                       cTTCACCTTGAAAAAACTACAT‑TTTTTTTCATNGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTANccc                  >  2:1325293/1‑76 (MQ=255)                                                        ttCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 >  2:97169/1‑76 (MQ=255)                                                         tCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                <  1:546903/76‑1 (MQ=255)                                                          cACCTTGAAAAAACTACATTTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                <  2:790827/76‑1 (MQ=255)                                                           aCCTTGAAAAAACTACAT‑TTTTTTTCATCGTGANATGGACAGAGGATAAATTGTTCNATAACGGATTNTCCCCGCt              >  2:1195036/1‑76 (MQ=255)                                                             cTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa            <  1:1181896/76‑1 (MQ=255)                                                               tGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:921349/76‑1 (MQ=255)                                                               tGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:899130/76‑1 (MQ=255)                                                                  aaaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       <  1:960981/76‑1 (MQ=255)                                                                   aaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      >  1:604023/1‑76 (MQ=255)                                                                    aaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc     >  2:987057/1‑76 (MQ=255)                                                                     aaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg    <  1:702463/76‑1 (MQ=255)                                                                      aCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc   >  1:395661/1‑76 (MQ=255)                                                                       cTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc  <  2:1126439/76‑1 (MQ=255)                                                                            |                                                                        TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑