| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,405 | +A | coding (155/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,405 | 1 | . | A | 93.8% | 173.3 / 3.8 | 48 | coding (155/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (24/21); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTT‑GTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCA > REL606/4202330‑4202473 | ccGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCtt < 2:897983/76‑1 (MQ=255)ccGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCtt < 1:583580/76‑1 (MQ=255) cGACCTGACGCACGCAACCCTGCTATTGCATNGTGGTTAGCAGGCGNTGGGTNGTGGAATTGGGTAGCCCGGCtta < 2:180974/76‑2 (MQ=255) aCCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCttagt > 2:1121833/1‑73 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTgc > 1:914381/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTgc > 1:659383/1‑76 (MQ=255) aCGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTgcgc < 1:1229670/76‑1 (MQ=255) cACGAAACCCTGCTGTTGCATTCTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAg > 1:7730/1‑76 (MQ=255) cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTT‑GTTGCGCCAGTTc > 1:1027169/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTcc < 1:747485/76‑1 (MQ=255) aCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGt < 1:1067833/76‑1 (MQ=255) cccTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTg < 2:1004671/76‑1 (MQ=255) ctgctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAg > 2:952819/1‑76 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGc > 1:1003720/1‑76 (MQ=255) ttGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCcac < 1:919154/76‑1 (MQ=255) atacATTGTGGTTNGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCcac < 1:181090/73‑1 (MQ=255) tGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCcaca > 2:595418/1‑76 (MQ=255) gCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCacac < 2:845088/76‑1 (MQ=255) cATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACt < 1:702334/76‑1 (MQ=255) aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTg < 1:1200194/76‑1 (MQ=255) aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTg < 2:417303/76‑1 (MQ=255) ttGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGc > 1:231066/1‑76 (MQ=255) ggTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCAAtt < 1:1107646/76‑1 (MQ=255) gCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAtt < 2:1104245/76‑1 (MQ=255) gCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAtt < 2:581530/76‑1 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCg > 1:726723/1‑76 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCg > 2:430238/1‑76 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTT‑GTTGCGCGAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 2:133085/1‑76 (MQ=255) gCGGTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGc < 1:1098070/76‑1 (MQ=255) gTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAAt > 1:1019641/1‑76 (MQ=255) gTGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAAt < 1:209274/76‑1 (MQ=255) tGGGTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATc > 2:870677/1‑76 (MQ=255) ggTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCa > 1:827190/1‑76 (MQ=255) ggTCGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGAATTCGGCAATCCa < 1:93004/76‑1 (MQ=255) cGTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTc > 2:977807/1‑76 (MQ=255) gTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTcc > 2:1064201/1‑76 (MQ=255) gTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTcc > 1:1259078/1‑76 (MQ=255) gTGGAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTCCCATTGGATTNGGCAATCCAccct > 1:186058/1‑73 (MQ=255) tGGAATTGGGTAACCCGGNTTAGTTGCGCCAGTTCCGTGAGTGCCAGACTGCCATTGGATTCGGCAATCCACTCCa > 1:1351843/1‑76 (MQ=255) ggAATTGGGTAACCCGGCTTAGCTGCGCCAGTTCCGTGAGTGCCACACTGCCATCGGATTCTGCAATCCACTCCAg > 2:206903/1‑76 (MQ=255) gAATTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGt < 2:765347/76‑1 (MQ=255) gAATTGGGTAACCCGGCTT‑GTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCNACTCCAGTa < 1:37858/76‑1 (MQ=255) aaTTGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTa > 2:757765/1‑76 (MQ=255) ttGGGTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAt > 1:1014564/1‑76 (MQ=255) gggTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAttt < 1:972959/76‑1 (MQ=255) gTAACCCGGCTTAGTTGTGCCNCTTCCNTGAGTGCCACACTGCCATTGNATACGGCAATCTACCCAAGTACTTTCa > 1:1378157/1‑76 (MQ=255) gTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCa > 2:258818/1‑76 (MQ=255) gTAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCa < 1:755100/76‑1 (MQ=255) tAACCCGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAg > 2:684391/1‑76 (MQ=255) aaCCCGGCTTAGTTGCGCCAGTTCCGTGAGAGCCACACTGCCATTGGATTCGGCAATCCACTNCAGTAATTTCAgg > 2:1294574/1‑76 (MQ=255) cccGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGcc < 2:827190/76‑1 (MQ=255) ccGGCTTAGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCa < 2:1065492/76‑1 (MQ=255) | CCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTT‑GTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCA > REL606/4202330‑4202473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |