Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,577,728	(GCTGGA)2→3	coding (740/1353 nt)	gor →	glutathione reductase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3577717 =	2 (0.040)	40 (0.980)	33/138	0.3	97.8%	coding (729/1353 nt)	gor	glutathione reductase
	?	REL606	= 3577722	0 (0.000)					coding (734/1353 nt)	gor	glutathione reductase

GTTGTCATTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3577795‑3577717 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tccagcTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGCCTTC  <  REL606/3577722‑3577649                                                                                                                                                           GTTGTCATTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCC                                                                               <  2:344807/76‑1  TTGTCATTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCA                                                                              <  2:634344/76‑1   TGTCATTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAG                                                                             <  2:269342/76‑1        TTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCA                                                                        >  2:168291/1‑76        TTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCA                                                                        <  2:149640/76‑1          GGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGC                                                                      >  1:983250/1‑76            CAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGT                                                                    >  2:1150966/1‑76             AGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTC                                                                   >  2:802711/1‑76             AGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTC                                                                   <  2:1376337/76‑1              GGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCA                                                                  <  1:1160670/76‑1               GCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAG                                                                 <  1:767314/76‑1                CTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGG                                                                <  2:527689/76‑1                  CGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCT                                                              >  2:978705/1‑76                  CGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCT                                                              >  2:747717/1‑76                  CGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCT                                                              >  2:472999/1‑76                  CGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCT                                                              >  2:546175/1‑76                   GCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTA                                                             <  1:1061381/76‑1                   GCGACCAATCGCCCAAATCAGGCAATCCACCGNTTGACTGCNACCATATTCCAGCTCCAGCNCCAGCGTCAGGCTA                                                             >  2:1182463/1‑76                    CGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTAC                                                            >  2:530981/1‑76                       CCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCAT                                                         <  2:399747/76‑1                               CCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTT                                                 <  2:628679/76‑1                               CCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTT                                                 >  2:717634/1‑76                                 AAATCAGGCAATCCACCGTTTCACTGCGTCCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTT                                               <  2:149716/76‑1                                 AAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGCCAGGCTACCATCGGCATTTTT                                               <  2:149710/76‑1                                        GCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACC                                        <  2:716021/76‑1                                         CAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCG                                       >  2:267166/1‑76                                             CCACCGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTT                                   <  1:775807/76‑1                                              CACCGTTTCNCTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTC                                  >  1:33596/1‑76                                                 CGTTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGG                               <  2:546609/76‑1                                                   TTTCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGAT                             <  1:1166334/76‑1                                                     TCACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCACTTGTCACTACCGCTTTCGGGATGG                           >  1:1343977/1‑76                                                      CACTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGC                          >  1:639964/1‑76                                                        CTGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGT                        >  2:952735/1‑76                                                         gGCGACCGTCTCCCAGCTCCGCCGCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATTGCGTT                       <  2:450736/75‑1                                                         TGCGACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTT                       <  1:1190187/76‑1                                                            GACCATCTTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGT                    <  1:454132/76‑1                                                                  CTTCCAGCTCCAGCTCCAGCGTCAGTCTACCATCGGCATTNNTCACTACCGCNNTTGGGATGGCGTTGGTGTGCAG              >  1:1280993/1‑76                                                                   TTCCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCANTANCGCTTTCGGGATGGNGTTGGTGTGCAGC             >  2:1281283/1‑76                                                                     CCAGCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTG           >  2:84429/1‑76                                                                        GCTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGG        >  2:991921/1‑76                                                                         CTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGG       >  2:745572/1‑76                                                                         CTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGG       >  2:947511/1‑76                                                                         CTCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGG       >  2:656048/1‑76                                                                          TCCAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGC      <  2:374349/76‑1                                                                            CAGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGCCT    <  1:680740/76‑1                                                                             AGCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGCCTT   <  2:846313/76‑1                                                                              GCTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGCCTTC  <  2:12591/76‑1                                                                                                                                                           GTTGTCATTGGCAGGCTCGCGACCAATCGCCCAAATCAGGCAATCCACCGTTTCACTGCGACCATCTTCCAGCTCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3577795‑3577717 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tccagcTCCAGCGTCAGGCTACCATCGGCATTTTTCACTACCGCTTTCGGGATGGCGTTGGTGTGCAGCTGCGGGCCTTC  <  REL606/3577722‑3577649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.