| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 21.7% | 175.3 / 28.0 | 60 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (28/19); new base T (13/0); total (41/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT > REL606/1747828‑1747978 | cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTGCAgg < 2:1401301/76‑1 (MQ=255) agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGc < 1:898827/76‑1 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCCGGCa > 2:59720/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:401562/1‑76 (MQ=255) gCTGATAATGTTNTTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:12933/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:935024/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcat > 1:684629/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcac > 1:62440/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:1206555/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:772732/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:1152683/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:540211/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 1:1188461/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 2:574189/1‑76 (MQ=255) tGATAATGTNATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtg > 1:30144/1‑75 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCGGCCATTACACGATGAACGCTTATGAACTCCNGTCAcatc > 1:1257390/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTcc < 1:824989/76‑1 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTcc > 2:1709/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 2:1133902/1‑76 (MQ=255) aTAATGTTANTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:33683/1‑73 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 2:696754/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc < 2:160105/76‑1 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTGTGAACTCCAGGCACTCCGCCa > 1:1338011/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 1:524784/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat < 2:109606/76‑1 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACTTAGGCatatc > 1:814487/1‑75 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 1:611954/1‑76 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt < 2:729945/76‑1 (MQ=255) ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttttt > 2:759462/1‑76 (MQ=255) ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttttt > 1:482804/1‑76 (MQ=255) ttatATTTTATTCTTTATTTCATGCAGCCATTNCAAGATGANCGCTTATGAACTCCAGGCANTCCGCCATAttttt > 2:1182771/1‑76 (MQ=255) atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGc > 1:597203/1‑76 (MQ=255) tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGcc > 1:1057691/1‑76 (MQ=255) tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGcc > 1:756653/1‑76 (MQ=255) ttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt > 1:215074/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg > 2:444139/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg < 1:1140806/76‑1 (MQ=255) tttttttttTCATGCAGCCATTACACGATGAACGCTTATGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTa < 1:1233444/73‑1 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa < 1:778684/76‑1 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa < 1:605664/76‑1 (MQ=255) tGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt < 1:361712/76‑1 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATTAACTGCAGGCACTCCGCCATATTTTTGCCATGACTAtt < 2:1225321/76‑1 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 1:1076483/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 2:1268266/1‑76 (MQ=255) ttATTTCANGAAGCCATTANACGATGAACGCTTATGAACTCCNGGCACTCCGCCATATTTTTGCCATGACTATtga < 2:1188461/76‑1 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:578435/1‑76 (MQ=255) tttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatga > 1:1161812/1‑76 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa > 1:301048/1‑76 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa > 1:331639/1‑76 (MQ=255) tCATGCAGCCATTACACGATGAACGCTTATGCACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt < 2:227906/76‑1 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc < 1:383198/76‑1 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc < 1:215187/76‑1 (MQ=255) gCCATTACACGATGAACGCTTATGACCTCCGGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc < 1:557915/76‑1 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt < 1:584075/76‑1 (MQ=255) aTTACACGATGAACGCTTATGACCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg < 1:519313/76‑1 (MQ=255) aTTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg > 1:674870/1‑76 (MQ=255) tACACGATGAACNCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa > 1:15258/1‑76 (MQ=255) cacGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt < 2:674287/76‑1 (MQ=255) acGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg > 1:1082819/1‑76 (MQ=255) cGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 1:828371/76‑1 (MQ=255) gCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg < 2:773466/76‑1 (MQ=255) tttATGAACTCCAGCCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg < 1:1016272/75‑1 (MQ=255) aTGACCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa < 1:444332/76‑1 (MQ=255) aTGAACTCCAGGCACTCCGCCATATTTNTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa > 1:1418766/1‑76 (MQ=255) ccccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTg < 1:943689/74‑1 (MQ=255) cccAGGCACTCCGCCATATTTTTGACATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa < 1:631918/75‑1 (MQ=255) ccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa > 2:463616/1‑76 (MQ=255) gCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt < 1:1139185/76‑1 (MQ=255) gCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt < 1:649886/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT > REL606/1747828‑1747978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |