Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,224	A→G	L361P (CTG→CCG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,224	0	A	G	100.0%	153.0 / NA	38	L361P (CTG→CCG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)

TGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGC  >  REL606/474151‑474294                                                                          |                                                                       tGCGACCAGTCCCGGGTTGAGAAAAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGt                                                                      <  1:900132/76‑1 (MQ=255)  gCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTa                                                                     >  2:164751/1‑76 (MQ=255)   cGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTaa                                                                    >  2:797525/1‑76 (MQ=255)    gAACAGTGCCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTaaa                                                                   <  2:1070453/76‑1 (MQ=255)    gAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTaaa                                                                   <  1:800018/76‑1 (MQ=255)         gTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCaa                                                              >  1:925465/1‑76 (MQ=255)             cGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGGAAATTCAATGcc                                                          >  2:473475/1‑76 (MQ=255)                gTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCAtt                                                       <  2:592218/76‑1 (MQ=255)                 ttGGGCAAAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTc                                                      <  1:386413/76‑1 (MQ=255)                 ttGAGCATAATCGACAGGATCGCCCCTGCCAGTACCNGGTTTTGTCCGGCCTACGGCGGTAAATTCAATGCNATTc                                                      >  1:169310/1‑76 (MQ=255)                 ttGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTc                                                      <  1:107268/76‑1 (MQ=255)                   gAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCCGCCCGCGGCGGGCAATTAAAAGGCATTtcc                                                    >  1:345298/1‑76 (MQ=255)                    aGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCa                                                   <  1:824112/76‑1 (MQ=255)                    aGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCa                                                   >  2:1042799/1‑76 (MQ=255)                        tAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTcc                                               >  2:1308813/1‑76 (MQ=255)                            cGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGcc                                           >  1:1061487/1‑76 (MQ=255)                            cGACAGGATCGCCCCTGCCAGTACCAGGTTTAGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGcc                                           <  2:231009/76‑1 (MQ=255)                                  gATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATa                                     >  2:1235516/1‑76 (MQ=255)                                   aTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTNAATTCAATGCCATTCCCAGTCCCGCCAGGATaa                                    <  2:1198688/76‑1 (MQ=255)                                   aTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCTATTCCCAGTCCCGCCAGGATaa                                    <  1:4259/76‑1 (MQ=255)                                    tCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATaaa                                   >  1:1140642/1‑76 (MQ=255)                                    tCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATaaa                                   <  1:145572/76‑1 (MQ=255)                                      cccccTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAAcg                                 <  1:964063/75‑1 (MQ=255)                                          cTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGaa                             >  2:842904/1‑76 (MQ=255)                                           tGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCCGGATAAACGCGAAc                            >  1:905269/1‑76 (MQ=255)                                           tGCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAAc                            >  2:1248631/1‑76 (MQ=255)                                            gCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTNAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAAcc                           >  2:1203748/1‑75 (MQ=255)                                            gCCAGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACt                           >  2:1264024/1‑76 (MQ=255)                                               aGTACCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCAc                        >  1:918911/1‑76 (MQ=255)                                                gtcccAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCAcc                       <  1:975727/73‑1 (MQ=255)                                                 tACCAGGTTTTGTCCGGCCTGCGGCGGGAAATTCAATGCCATTCCCCGTCCCGCCAGGAGAAAAGAGAAGTCACCa                      >  2:351194/1‑76 (MQ=255)                                                  aCCAGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCaa                     <  1:867075/76‑1 (MQ=255)                                                     aGGTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCt                  <  1:349958/76‑1 (MQ=255)                                                      ggTTTTGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTg                 <  1:361665/76‑1 (MQ=255)                                                          ttGTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGcc             >  1:1083043/1‑76 (MQ=255)                                                            gTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAg           <  1:473475/76‑1 (MQ=255)                                                            gTCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAg           >  1:1199724/1‑76 (MQ=255)                                                             tCCGGCCTGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAgg          <  1:944559/76‑1 (MQ=255)                                                               cGGCCTGCGGCGGTAAATTCAATGCCATTCCTAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCt        <  2:230700/76‑1 (MQ=255)                                                                    tGCGGCGGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTggcgg   >  1:497385/1‑76 (MQ=255)                                                                     gcggcgGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTggcggc  >  2:1258267/1‑76 (MQ=255)                                                                          |                                                                       TGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGC  >  REL606/474151‑474294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑