| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,157,919 | C→T | G340S (GGC→AGC) | rne ← | fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,157,919 | 0 | C | T | 95.7% | 72.9 / ‑2.9 | 24 | G340S (GGC→AGC) | rne | fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/9); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
AGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTC > REL606/1157849‑1157991 | aGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGt > 2:541715/1‑76 (MQ=255) gTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATGACAATCAGGCCGCTGAGGTCACGc > 1:1341283/1‑76 (MQ=255) ttttCTACCGCACGCTGGTGGCGTACTGACGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCa > 1:1173202/1‑76 (MQ=255) ttCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAgg > 1:1269679/1‑76 (MQ=255) tACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGgcgc > 2:1390220/1‑76 (MQ=255) tACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGgcgc > 2:153655/1‑76 (MQ=255) gccGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCa < 1:305829/75‑1 (MQ=255) aCCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCa < 1:1017998/76‑1 (MQ=255) gCACGCTGGTGGCGTACTGGCGTCACGTCGACGAAGTCGCNNACAATCAGGCNNCGGAGGTCACGCAGGCGCAGCt > 1:1281179/1‑76 (MQ=255) cACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTg < 1:582474/76‑1 (MQ=255) cGTACTGGCGTCATGTCGATGAAGTCCATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAAtct > 1:349707/1‑76 (MQ=255) gTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAAtctc > 1:755587/1‑76 (MQ=255) aCTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCAt > 1:231342/1‑76 (MQ=255) cTGGCGTCATGACGATGAAGGCGATAACAATCAGGCGCCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATc < 1:600728/76‑1 (MQ=255) cGTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCa > 1:707289/1‑76 (MQ=255) gTCATGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAg < 1:1177542/76‑1 (MQ=255) ctGTCGATGAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCtt < 2:60431/75‑1 (MQ=255) gAAGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTa < 2:977815/76‑1 (MQ=255) aaGTCGATAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAg < 2:1121308/76‑1 (MQ=255) aTAACAATCAGGCCGCTGAGGTTACGCAGGCGCAGCTGACGAGCAATCTCATCGTCAGCTTGGAGGGTAGTGTTaa > 2:212187/1‑76 (MQ=255) aTAACAATCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTaa < 1:1109219/76‑1 (MQ=255) cAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgc > 2:958423/1‑76 (MQ=255) aaTCAGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGCGGCTAGTGTTAAAagcg > 2:1242885/1‑76 (MQ=255) aGGCCGCTGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGGTAGTGTTAAAAGCGGgtt > 1:657776/1‑76 (MQ=255) gccgctGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttct < 1:1177750/76‑1 (MQ=255) ccgctgAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTTAACGCGGTttctt > 1:343674/1‑76 (MQ=255) cgctgAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc > 1:1102836/1‑76 (MQ=255) | AGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTC > REL606/1157849‑1157991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |