Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	124.3 / NA	36	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/17);  total (19/17)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGC  >  REL606/2193706‑2193846                                                                          |                                                                    gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                   <  2:352441/76‑1 (MQ=255) gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                   <  1:569385/76‑1 (MQ=255) gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                   >  1:282379/1‑76 (MQ=255)   cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                 >  1:295027/1‑76 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                >  2:617435/1‑76 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                             >  1:754610/1‑76 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                             <  1:243973/76‑1 (MQ=255)        cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAAAGCCAATCCCCACCAg                                                            >  2:86203/1‑76 (MQ=255)         aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCAACAGa                                                           >  2:236894/1‑76 (MQ=255)           ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                         <  2:247717/76‑1 (MQ=255)            cGGCCTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                        <  2:464174/76‑1 (MQ=255)                         cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                           <  2:721447/76‑1 (MQ=255)                              ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                      <  1:1388587/76‑1 (MQ=255)                              ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                      <  1:1263092/76‑1 (MQ=255)                                tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                    >  1:421091/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                   >  1:1068032/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                   >  2:868124/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                   >  2:6968/1‑76 (MQ=255)                                  tGGCTGTCGTAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                  <  1:45091/76‑1 (MQ=255)                                     cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                               >  1:1041590/1‑76 (MQ=255)                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGTCTGTATTAAACAGGCtt                             >  1:151736/1‑76 (MQ=255)                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                            <  2:38334/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                          <  2:1161057/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                          <  2:1041590/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                          <  2:749875/76‑1 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                         <  2:1264820/76‑1 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                        >  2:263020/1‑76 (MQ=255)                                               tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACAGa                     >  2:885848/1‑76 (MQ=255)                                                cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                    >  2:349688/1‑76 (MQ=255)                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                <  1:647858/76‑1 (MQ=255)                                                       aaTAACAGNAGAACGCCAANCCCCACCAGATAAAGCATGACGNCTGTATTAAACAGGCTTTTTACCGATGGCGCAt             <  2:1188655/76‑1 (MQ=255)                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt             <  1:1126697/76‑1 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc            >  2:552790/1‑76 (MQ=255)                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc          >  2:1379646/1‑76 (MQ=255)                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg         <  2:1140971/76‑1 (MQ=255)                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg         >  1:882921/1‑76 (MQ=255)                                                               cAAAACGCCAATCCCCATCAGAGAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGAGTAcc     >  2:208226/1‑76 (MQ=255)                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa    >  2:779958/1‑76 (MQ=255)                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc  >  2:367241/1‑76 (MQ=255)                                                                          |                                                                    GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGC  >  REL606/2193706‑2193846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑