Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,373,769	C→T	G93S (GGT→AGT)	rplF ←	50S ribosomal protein L6

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,373,769	0	C	T	100.0%	212.8 / NA	54	G93S (GGT→AGT)	rplF	50S ribosomal protein L6
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

GCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACG  >  REL606/3373697‑3373841                                                                         |                                                                         gCTGATTGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCAGATTGCCTTTAACCGCTGCACGGTAACTTac                                                                       <  1:1300365/76‑1 (MQ=255) gCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTac                                                                       <  1:1153881/76‑1 (MQ=255)   tGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTacac                                                                     <  1:901269/76‑1 (MQ=255)   tGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTacac                                                                     >  1:584480/1‑76 (MQ=255)         tCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACaccaacca                                                               >  2:113280/1‑76 (MQ=255)         tCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACaccaacca                                                               <  1:307823/76‑1 (MQ=255)          cAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAg                                                              >  1:509119/1‑76 (MQ=255)          cAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAg                                                              <  1:213539/76‑1 (MQ=255)           aaCAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGc                                                             >  1:385481/1‑76 (MQ=255)            aCAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCt                                                            <  1:865775/76‑1 (MQ=255)             cAGGATGAGAGAAACCCAGAGCCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTg                                                           <  1:1342007/76‑1 (MQ=255)             cAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTg                                                           >  2:966627/1‑76 (MQ=255)              aGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGc                                                          >  1:5770/1‑76 (MQ=255)              aGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGc                                                          <  1:1241604/76‑1 (MQ=255)                      agaAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACANCAACCAGCTGCAGcttctt                                                  <  1:1321077/76‑1 (MQ=255)                      agaAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGcttctt                                                  <  2:707059/76‑1 (MQ=255)                      agaAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGcttctt                                                  <  1:481008/76‑1 (MQ=255)                       gaAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTa                                                 >  2:113323/1‑76 (MQ=255)                         aaCCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGt                                               <  1:353407/76‑1 (MQ=255)                          aCCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTg                                              <  1:381436/76‑1 (MQ=255)                            ccAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGaa                                            <  2:891662/76‑1 (MQ=255)                             cAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAg                                           >  1:774055/1‑76 (MQ=255)                               gagaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGcc                                         <  2:458618/76‑1 (MQ=255)                                agaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCt                                        >  2:528942/1‑76 (MQ=255)                                 gaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCtt                                       <  2:1148289/76‑1 (MQ=255)                                 gaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCtt                                       <  2:863538/76‑1 (MQ=255)                                 gaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCtt                                       >  2:999983/1‑76 (MQ=255)                                  aCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTc                                      >  1:1093958/1‑76 (MQ=255)                                  aCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTc                                      >  1:214070/1‑76 (MQ=255)                                   cAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTATACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCg                                     <  2:586218/76‑1 (MQ=255)                                    aGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCgg                                    >  2:101476/1‑76 (MQ=255)                                      gTTAATCACATTGCCTTTCACTGCTGCACGGTAACTTACACCAACCAGCGGCAGCTTCTGAGTGAAGCCTTCGGTa                                  <  1:1339695/76‑1 (MQ=255)                                       ttAATCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACAAGCTGCAGCTTCTTAGTGAAGCCTTCGGTaa                                 >  2:870136/1‑76 (MQ=255)                                         aaTCACATTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGATTCTTAGTGAAGCCTTCGGTAaca                               >  1:1336751/1‑76 (MQ=255)                                            cacaTTGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCg                            <  2:169630/76‑1 (MQ=255)                                                ttGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATaa                        >  2:909862/1‑76 (MQ=255)                                                ttGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATaa                        <  1:646713/76‑1 (MQ=255)                                                ttGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATaa                        >  2:836473/1‑76 (MQ=255)                                                 tGCCTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAAc                       <  1:255459/76‑1 (MQ=255)                                                   ccTTTAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCa                     >  1:792520/1‑76 (MQ=255)                                                    cTTTAACCGCTGNACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCAt                    >  1:17251/1‑76 (MQ=255)                                                      ttAACCGCTGCACGGTAACTTACACCAACCAGNTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCNTTg                  >  2:1323742/1‑76 (MQ=255)                                                      ttAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTg                  <  2:427896/76‑1 (MQ=255)                                                      ttAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTg                  <  1:56586/76‑1 (MQ=255)                                                       tAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGa                 <  2:1262648/76‑1 (MQ=255)                                                       tAACCGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGa                 >  2:989297/1‑76 (MQ=255)                                                          ccGCTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGtt              >  2:840101/1‑76 (MQ=255)                                                             cTGNACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAGCACCGATAACCATTGAGTTtag           <  2:1289371/76‑1 (MQ=255)                                                             cTGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTcag           <  1:277367/76‑1 (MQ=255)                                                              tGCACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTcagc          >  1:1046624/1‑76 (MQ=255)                                                                cACGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTcagcag        <  1:714523/76‑1 (MQ=255)                                                                  cGGTAACTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAggg      <  1:5014/76‑1 (MQ=255)                                                                    gTAACTTACACCAACCCGCTGCAGCTGCTTAGTGAAGCCTTCGGTAACACCCATTACTATTGAGTCCAGCAGGGCa    >  1:1334581/1‑76 (MQ=255)                                                                      aaCTTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACNGATAACCATTGAGTTCAGCAGGGCAcg  <  1:1421971/76‑1 (MQ=255)                                                                         |                                                                         GCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACG  >  REL606/3373697‑3373841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑