| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,991 | (T)5→4 | intergenic (‑192/‑131) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,987 | 0 | T | . | 91.5% | 191.4 / 14.5 | 47 | intergenic (‑188/‑135) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base . (23/20); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTA > REL606/3911912‑3912057 | aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattttat > 2:1407735/1‑76 (MQ=255) ncGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCNCTCACtattttatt > 2:220239/2‑76 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattttattt > 2:838290/1‑76 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTattttatttt < 2:506175/76‑1 (MQ=255) aaaGGATGAACTANGAGGAAGGGAACAACAATCATACTGAAATTGTATTTTTTTCACTCACTATTTTATTTTaaaa < 2:1403582/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTaaaa > 1:777579/1‑76 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTAaaaa < 2:1418856/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTCCACTCACTATNTTATTTTaaagag > 1:206334/1‑73 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaa > 2:841827/1‑76 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 1:1024757/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 2:366981/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa < 1:993952/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta < 1:1478594/76‑1 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtat > 2:767539/1‑76 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtata > 2:702468/1‑76 (MQ=255) ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATAtt < 1:678985/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa > 2:415145/1‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa < 1:992251/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa < 1:291297/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGaa < 2:508031/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGaa > 2:1136424/1‑76 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAat < 2:470363/76‑1 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatt < 1:1407736/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatta > 2:80104/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattat < 1:860439/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTCACNCACTATTTTA‑TTTTAAAAAACANCAATTTATATNGAAattat > 2:1289183/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAAttatta > 2:968453/1‑76 (MQ=255) acaTTCATACTGAGATTGAATTTCTTTCACTCACTATTTTA‑TTTTAAAAAACAAGAATTTATATTGAAATTATTaa < 1:1318560/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaa < 1:335570/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaa > 2:664759/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCNCTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa > 1:1522186/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa > 1:224122/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:398426/76‑1 (MQ=255) caTTCATACTGAAATGGAATTTGTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 2:53267/76‑1 (MQ=255) ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 2:1420063/76‑1 (MQ=255) ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 2:701038/76‑1 (MQ=255) cATACTGAAATTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 1:1470355/1‑76 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 2:1210531/1‑76 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:1113332/1‑76 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:810332/76‑1 (MQ=255) gAATTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg < 1:1136518/76‑1 (MQ=255) tttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa > 2:24598/1‑76 (MQ=255) tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAGTTATTAAACGCATCATAAAAATCGGCCaaaa > 1:614591/1‑76 (MQ=255) tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa > 1:313909/1‑76 (MQ=255) ttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa > 2:827831/1‑76 (MQ=255) cactcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat > 1:1013814/1‑76 (MQ=255) cactcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat > 1:669899/1‑76 (MQ=255) ctcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAANGCATCATAAAAATCGGCCAAAAAatat < 1:1533442/76‑1 (MQ=255) tcactATTTNA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATc > 1:33159/1‑76 (MQ=255) cactATTTTA‑TTTTAAACAACAGCAATTTATATTGAAATTATTAAACGCATCATAAAAATCTGCCAAAAAATATCt > 2:233652/1‑76 (MQ=255) actATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 1:275670/1‑76 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 2:654275/76‑1 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 1:747607/76‑1 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 1:386306/76‑1 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt > 1:240879/1‑76 (MQ=255) atttta‑ttttAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:737319/1‑76 (MQ=255) | AGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTA > REL606/3911912‑3912057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |