| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,195,874 | 0 | A | C | 31.8% | 120.7 / 14.9 | 63 | T318P (ACC→CCC) | aceB | malate synthase |
| Reads supporting (aligned to +/- strand): ref base A (34/9); new base C (0/20); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAAT > REL606/4195802‑4195948 | gTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCt > 1:1158613/1‑76 (MQ=255)gTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCt < 2:1173365/76‑1 (MQ=255) ccTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGc < 2:260351/76‑1 (MQ=255) tGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGGATGCTTACTCACGCCTGTTGATTAAAACCTGCCa < 2:1349247/76‑1 (MQ=255) tGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCa > 1:1184167/1‑76 (MQ=255) tGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCa > 1:471404/1‑76 (MQ=255) gCCAGACAGGCAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCAt < 1:545506/76‑1 (MQ=255) cagacagaCAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATaa > 1:1237061/1‑76 (MQ=255) agacagacGGGCAGTGACGAGGGATAAACCATTCCTGAATGCTTACCCCCGCCTGTTGATTAAACCCTGCCATaaa < 2:432993/76‑1 (MQ=255) acagacagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcg > 1:1199787/1‑76 (MQ=255) agccagGCAGTGACGATGGATACACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATACAngcg > 2:1389085/4‑76 (MQ=255) acagGCAGTAACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGt > 2:114789/1‑76 (MQ=255) agGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGc > 2:1366149/1‑76 (MQ=255) gCAGGGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCtt < 2:1152895/76‑1 (MQ=255) ctgTGACGATGGATAACCCATCCCTGAATGCTTACTCCCGCTTGTTGATTAAAACCTGCCATAAACGCGGTGCttt < 2:371589/74‑1 (MQ=255) cAGTGACGATGGATAACCCATTCCTGAAGGCTTACCCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCttt < 2:347139/76‑1 (MQ=255) cAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCttt < 2:800937/76‑1 (MQ=255) cAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCttt < 1:1501986/76‑1 (MQ=255) cAGTGACGATGGATAAACCATTCCTGAATGCCTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCttt < 2:242171/76‑1 (MQ=255) cAGTGACGATGAAAAAACCATTCCTGAATGCTTACTCACGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCttt < 2:1027679/76‑1 (MQ=255) cAGNGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTNTTGATTAAACCCTGCCATAAACGCGGTGCttt < 2:1434453/76‑1 (MQ=255) cAGGGACGATGGAAAACCCATCCCTGAATGCTTCCCCACGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCttt < 1:902310/76‑1 (MQ=255) aGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCtttt < 1:487817/76‑1 (MQ=255) aGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTACCATAAACGCGGTGCtttt > 2:350278/1‑76 (MQ=255) gTGACGATGGATAAACCATTCCTGAATGCTTCCTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTg < 1:597377/76‑1 (MQ=255) gTGACGATGGATAAACCATCCCTGAATGCTTACTCACGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTg < 1:1297295/76‑1 (MQ=255) tGACGATGGATAACCCATTCCTGAATGCTTACCCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGc < 1:1239938/76‑1 (MQ=255) tGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGc < 2:847576/76‑1 (MQ=255) naCGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAANGCGGTGCTTTTGCg > 2:211284/2‑76 (MQ=255) ggcGAGGGATAAACCATCCCGGAAGCCTTACCCCCGCCTGTTAATAAAACCCGGCCATAAACGCGGTGCTTTTGCg < 1:633502/74‑1 (MQ=255) gACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCg < 1:1018455/76‑1 (MQ=255) gATGGATAACCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATg < 1:955815/76‑1 (MQ=255) aTGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATgg > 2:635682/1‑76 (MQ=255) ggAAAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCNTTTGCGATGggc < 1:15052/76‑1 (MQ=255) aaaCCATTCCTGAATGCTTACTCACGCCTGTTGNTTAAAACCTGCCATAAACGCGGNGCTTTTGCTANGGGCGGCa > 2:1281483/1‑76 (MQ=255) aaaCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCa > 2:1353865/1‑76 (MQ=255) aaaCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCa > 2:1077717/1‑76 (MQ=255) aaCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCAt > 1:280480/1‑76 (MQ=255) aaCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCAt > 1:280489/1‑76 (MQ=255) ccATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATgg > 1:574322/1‑76 (MQ=255) ccTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGgcggcg > 1:1483043/1‑76 (MQ=255) ccTGAATGCTTACTCACACCTGCTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGCGCGGCATGgcggcg > 2:233933/1‑76 (MQ=255) tGAATGCTTCCTCACCCCTGTTGTTTAAACCCTGCCATAAGCGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGtt < 1:721090/76‑1 (MQ=255) tGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGtt > 1:367564/1‑76 (MQ=255) gCTTACTCACGCCTGTTGATGATAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTc > 1:6865/1‑76 (MQ=255) ttACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg > 1:1234461/1‑76 (MQ=255) ttACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg > 1:976204/1‑76 (MQ=255) ttACTCACGCCTGTTAATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg < 2:659269/76‑1 (MQ=255) aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAg > 1:739363/1‑76 (MQ=255) cTCACGCATGTTGATTAAACGCNNCCATAAACGCNNCGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGc < 1:1378959/76‑1 (MQ=255) ccccGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCa < 1:1008573/73‑1 (MQ=255) ccACGCCTGTTAATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCa < 1:1234824/75‑1 (MQ=255) cccGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa < 2:1525300/74‑1 (MQ=255) cACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa > 1:829843/1‑76 (MQ=255) cACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa > 1:1107587/1‑76 (MQ=255) cACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa > 1:298522/1‑76 (MQ=255) aCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaaa > 1:218655/1‑76 (MQ=255) ccTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGNATGGCGGCGTTTATTCCGAGCAAAGAt < 1:1527209/76‑1 (MQ=255) cttttGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg < 2:541858/73‑1 (MQ=255) cTGTTGATTAAACTCTGCCATAAGCGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg < 1:1314982/76‑1 (MQ=255) tGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGa > 2:295411/1‑76 (MQ=255) tGTTAATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGa < 2:374330/76‑1 (MQ=255) tttGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTNTATTCCGAGCAAAGATGaa < 1:1427090/75‑1 (MQ=255) nttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTNCGAGCAAAGATGaa > 2:209384/2‑76 (MQ=255) ttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAag > 1:311372/1‑76 (MQ=255) tGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga > 1:369725/1‑76 (MQ=255) tGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga < 2:1124375/76‑1 (MQ=255) tgttAAACCCTGCCNTAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAagan < 2:222286/74‑2 (MQ=255) gATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAagag > 1:700323/1‑76 (MQ=255) ataaaacCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGc < 2:473841/73‑1 (MQ=255) aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGc > 1:790708/1‑76 (MQ=255) aaaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcaca > 2:1481694/1‑76 (MQ=255) aaaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGGTTATTCCCAGCAAAGATGAAGAGgaca > 2:769313/1‑76 (MQ=255) aaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACaa > 2:38963/1‑76 (MQ=255) aaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAAt > 2:1054474/1‑76 (MQ=255) | GTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAAT > REL606/4195802‑4195948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |