| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 430,954 | G→A | E4E (GAG→GAA) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 430,954 | 0 | G | A | 98.0% | 185.8 / ‑3.6 | 52 | E4E (GAG→GAA) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (29/21); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
ATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCC > REL606/430880‑431026 | aTGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGc < 1:445834/76‑1 (MQ=255) cGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTc > 2:1406521/1‑76 (MQ=255) tGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTg > 2:410983/1‑76 (MQ=255) gAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGa > 2:705206/1‑76 (MQ=255) gAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATACTGAACGTTCTGa > 1:1315535/1‑76 (MQ=255) aaGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGaa > 1:483254/1‑76 (MQ=255) aGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAAc < 1:1502626/76‑1 (MQ=255) cacaGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGc > 2:495758/1‑76 (MQ=255) gTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTg < 1:992715/76‑1 (MQ=255) gtgtCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGaaa > 1:74465/1‑76 (MQ=255) gATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTAt < 1:1334481/76‑1 (MQ=255) gATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTAt > 1:977268/1‑76 (MQ=255) aTTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTAtt > 1:846938/1‑76 (MQ=255) ccTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCg > 2:566933/1‑76 (MQ=255) ccTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCg < 1:604910/76‑1 (MQ=255) cTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGc > 1:1397576/1‑76 (MQ=255) tGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCt < 2:921391/76‑1 (MQ=255) cGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTgcg > 2:82678/1‑76 (MQ=255) cGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTgcg < 1:706428/76‑1 (MQ=255) ggAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTgcgc < 1:498339/76‑1 (MQ=255) ggAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTgcgc < 1:470445/76‑1 (MQ=255) aaaTTAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGa < 2:1359808/76‑1 (MQ=255) ttAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGAtgt < 1:849422/76‑1 (MQ=255) ttAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGAtgt > 1:592789/1‑76 (MQ=255) tAAACTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGAtgtg > 2:725324/1‑76 (MQ=255) tAAACTAAGAGAGAGCTCNATGAATCCTGAACGTTCTGATCGCATTAAAATCCCCGTATTGCCGCTGCGCGAtgtg > 1:1466302/1‑76 (MQ=255) aaaCTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATgtgg > 2:603933/1‑76 (MQ=255) aaaCTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATgtgg > 2:831740/1‑76 (MQ=255) aaaCTAAGACAGAGCTCTATGAANCCTGANCGTTCTGAACGCATNGAGATCACCGTATTGCTGCTGCGCGATgtgg > 2:206534/1‑76 (MQ=255) aCTAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATgtggtg > 2:1407023/1‑76 (MQ=255) tAAGAGAGAGCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTggtggt > 2:937908/1‑76 (MQ=255) tAAGAGAGAGCTCTATGAATCCTGAACGTTCCGAACGCATNNAAATCCCCGTNNTACCGCTGCGCGATGTggtggt > 1:1372229/1‑76 (MQ=255) agagagagCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGttt < 1:763247/76‑1 (MQ=255) agagagagCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGACGTGGTGGttt > 2:951938/1‑76 (MQ=255) agagagCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTAt < 1:748497/76‑1 (MQ=255) gagCGCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCg > 1:86266/1‑76 (MQ=255) gCTCTATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGca > 1:545512/1‑76 (MQ=255) ctctATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGcac > 2:1154465/1‑76 (MQ=255) tctATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGAGATGTGGTGGTTTATCCGcaca < 1:9110/76‑1 (MQ=255) tATGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATg > 1:1100314/1‑76 (MQ=255) aTGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATgg < 2:953297/76‑1 (MQ=255) tGAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGt < 2:168336/76‑1 (MQ=255) gAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTc < 1:495451/76‑1 (MQ=255) gAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTc < 2:910365/76‑1 (MQ=255) gAATCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTc > 1:698765/1‑76 (MQ=255) gAATCCTGAACGTNCTGAACGCATTGAAATGCCCGTATTGCCGCTTCGCGATGTGGTGGTTTATCCGCACATGGTc < 2:1397575/76‑1 (MQ=255) aTCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCAt < 1:494603/76‑1 (MQ=255) aTCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTAAt > 2:611872/1‑76 (MQ=255) tCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATc < 1:659956/76‑1 (MQ=255) tCCTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATc > 1:964649/1‑76 (MQ=255) cTGATCCTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATccc > 1:84223/1‑76 (MQ=255) cTGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGNGGTGGTTTATCCGCACATGGTCATccc < 1:1525796/76‑1 (MQ=255) tGAACGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATcccc > 1:598281/1‑76 (MQ=255) | ATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCC > REL606/430880‑431026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |