| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,160,666 | T→G | intergenic (+130/‑190) | rplL → / → rpoB | 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,160,666 | 0 | T | G | 93.3% | 173.7 / ‑1.9 | 45 | intergenic (+130/‑190) | rplL/rpoB | 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (22/20); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ACCAGCCTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAG > REL606/4160595‑4160738 | cncNGCCTTTNTGCGCTGTAAGGCGCCAGTAGCGTATCACACTNTATGACTACTGCTATNCCTTTCACTGCGTGtt < 1:112372/74‑1 (MQ=255)ccgaGCCTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGtt < 2:885255/73‑1 (MQ=255) cAGCCTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTc < 2:1367839/76‑1 (MQ=255) gcccTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCt < 1:710439/74‑1 (MQ=255) aGCCTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATTCGTGTTTCt > 1:43896/1‑76 (MQ=255) cTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATc > 2:900763/1‑76 (MQ=255) cTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATc > 2:501979/1‑76 (MQ=255) tttttGCGCTGTAAGGCACCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATcg > 2:481154/1‑76 (MQ=255) tttGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATcgac > 1:561314/1‑76 (MQ=255) ttGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATcgacg > 2:61708/1‑76 (MQ=255) tGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATcgacga > 1:611200/1‑76 (MQ=255) gTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAat < 1:1224871/76‑1 (MQ=255) tAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAata > 1:385089/1‑76 (MQ=255) tAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAata > 2:371205/1‑76 (MQ=255) aGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAAtata > 2:1333873/1‑76 (MQ=255) gcNAGTCGCGTCTCACACTGTTTGAGTACTGCTGTGCCTTTCAATGCGTGTTTCTATCTACGACTTAATATTCTGc < 2:1442482/76‑1 (MQ=255) gcCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGc < 1:308969/76‑1 (MQ=255) cAGTAGCGTTTCACACTGTTTGACTACTGCAGTGCCTTTCAATGCGTGTTTCTATCGACGACNTAATATACTGCGa > 2:1401539/1‑76 (MQ=255) aGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGAc > 1:615870/1‑76 (MQ=255) tAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAg > 1:1273279/1‑76 (MQ=255) gTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATNCGTGTTTACANCGCCAACTTAATATACTGCGCCANGACg > 1:175403/1‑76 (MQ=255) gTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACg > 1:1040401/1‑76 (MQ=255) gTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACg > 1:1040391/1‑76 (MQ=255) tttCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGt > 2:144819/1‑76 (MQ=255) tttCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGt > 1:1005276/1‑76 (MQ=255) tttCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGt > 2:1091698/1‑76 (MQ=255) tCACACTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATAAACTGCGACAGGACGTcc > 2:1075065/1‑76 (MQ=255) cacaCTGTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTNTCGACGACTTAATATACTGCGACAGGACGTCCg < 2:1295857/76‑1 (MQ=255) cacaCTGTTTGACTACTGCTGTGCCTTTCAACGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCg < 2:245317/76‑1 (MQ=255) gTTTGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACNACTTAATATACTGCGACAGGACGTCCGTTCtgt < 2:1304262/76‑1 (MQ=255) ttGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTgtgt > 2:1337310/1‑76 (MQ=255) ttGACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTgtgt > 1:382725/1‑76 (MQ=255) gACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTaa < 2:936050/76‑1 (MQ=255) gACTACTGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTaa < 2:478229/76‑1 (MQ=255) actactGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTaaa > 2:1510790/1‑76 (MQ=255) ctactGCTGTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAAt < 1:402887/76‑1 (MQ=255) ctgctgTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATAGACTGCGACAGGACGTCCGTTCTGGGTAAATCGc < 2:1313829/76‑1 (MQ=255) ctgctgTGCCTTTCAATGCGTGTTTCTATAGACGACATAATATACTGCGACAGGACGNCCGTTCTGTGTAAATCGc < 1:1454175/76‑1 (MQ=255) tgctgTGCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCa < 1:501980/76‑1 (MQ=255) gtgCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGa > 1:1496580/1‑76 (MQ=255) tgCCTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGaa < 1:1194136/76‑1 (MQ=255) ccTTTCAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAAt < 2:814386/76‑1 (MQ=255) cAATGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGttt < 1:1091699/76‑1 (MQ=255) aaTGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTa < 1:563604/76‑1 (MQ=255) tGCGTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAg < 2:797608/76‑1 (MQ=255) | ACCAGCCTTTTTGCGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAG > REL606/4160595‑4160738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |