Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	475,031	G→T	A92E (GCG→GAG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	475,031	0	G	T	100.0%	97.3 / NA	28	A92E (GCG→GAG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/19);  total (9/19)

GGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCA  >  REL606/474958‑475103                                                                          |                                                                         gatCATTAGCGACCACACTAGCACGGCGGAGGGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCa                                                                        <  1:1389240/74‑1 (MQ=255)    cATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCg                                                                     <  1:1360337/76‑1 (MQ=255)     aTTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCgg                                                                    <  1:602199/76‑1 (MQ=255)      ttAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCggg                                                                   >  1:1265426/1‑76 (MQ=255)       tAACGACCAGCCCAGCAGGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCCCCGGGa                                                                  >  2:15099/1‑76 (MQ=255)         aCGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAAt                                                                >  2:110071/1‑76 (MQ=255)          cGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATg                                                               <  1:768504/76‑1 (MQ=255)               agcccagcACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGAt                                                          >  1:360886/1‑76 (MQ=255)               agcccagcACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGAt                                                          <  1:830160/76‑1 (MQ=255)                  ccagcACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGc                                                       <  2:1171386/76‑1 (MQ=255)                    agcACGGCGGAGAGCGCCGTGCCCAGCAGCGGCGCCACGGCTATCTGGGCGATCTCCCCGGGAATGGCGATGGCCt                                                     <  2:1182675/76‑1 (MQ=255)                     gcACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCtt                                                    <  2:31689/76‑1 (MQ=255)                      cACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATNTGGGCGATCTCACCGGGAACGGCGATGGCCttt                                                   <  2:1295795/76‑1 (MQ=255)                             gagagCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCa                                            >  2:727361/1‑76 (MQ=255)                                agCGCCATCCCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCa                                         <  2:190335/76‑1 (MQ=255)                                 gcgcCATACACAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCaa                                        <  1:46162/76‑1 (MQ=255)                                   gcCATACCCAGCAGTGTCGCCACGTCTAGCTNGGCGATCTTACCGGNAATGGNGATGGCCTTTACCACCATCAAAt                                      <  2:202831/76‑1 (MQ=255)                                     cATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATcc                                    >  2:528688/1‑76 (MQ=255)                                       gaCCCAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCtt                                  <  2:297721/75‑1 (MQ=255)                                         cccAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCa                                <  2:578659/76‑1 (MQ=255)                                         cccAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCa                                <  2:767110/76‑1 (MQ=255)                                          ccAGCAGCGTCGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAg                               >  1:421311/1‑76 (MQ=255)                                          ccAGCAGAGTCGCCGCGGCTATCTGGGCGATCTCTTCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAg                               <  2:497785/76‑1 (MQ=255)                                                    cGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGc                     <  1:556811/76‑1 (MQ=255)                                                    cGCCACGGCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGc                     <  2:465937/76‑1 (MQ=255)                                                          ggCTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCg               <  1:609455/76‑1 (MQ=255)                                                            cTATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGAc             >  1:826532/1‑76 (MQ=255)                                                             tATCTGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACg            >  2:1173404/1‑76 (MQ=255)                                                                 tGGGCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCaa        >  1:612793/1‑76 (MQ=255)                                                                  gggCGATCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCANACCGACGCCaaa       <  1:18460/76‑1 (MQ=255)                                                                       aTCTCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCa  >  1:120787/1‑76 (MQ=255)                                                                          |                                                                         GGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCA  >  REL606/474958‑475103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑