Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	649,985	C→G	V273L (GTG→CTG)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	649,985	0	C	G	100.0%	114.0 / NA	33	V273L (GTG→CTG)	mrdA	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/15);  total (18/15)

TTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATAT  >  REL606/649919‑650056                                                                   |                                                                        ttGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGcgcg                                                                >  2:607017/1‑76 (MQ=255)  tGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGCTCGGTTACTACCAGAGCTGCGCgg                                                               >  1:1452745/1‑76 (MQ=255)   gggTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGc                                                              >  1:828410/1‑76 (MQ=255)      tCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTAc                                                           >  2:682796/1‑76 (MQ=255)      tCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTAc                                                           >  2:361190/1‑76 (MQ=255)        aTAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTAccc                                                         >  2:1247777/1‑76 (MQ=255)          aaCTAGGCGTGGAAACCAGCGCCAGCACCCGACCTGTACGCGGATCGGTGTCTACCAGAGCTGCGCGGGTACCCgg                                                       >  2:721505/1‑75 (MQ=255)             tAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCcag                                                    >  1:956193/1‑76 (MQ=255)               ggCGTGGAAACCAGCGCCAGCCCCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCcagca                                                  <  2:974426/76‑1 (MQ=255)                gCGTGGAAACCAGCGCCAGCCCCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCcagcag                                                 <  2:1113545/76‑1 (MQ=255)                 cGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCagcagc                                                >  1:452917/1‑76 (MQ=255)                  gTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCg                                               >  1:547961/1‑76 (MQ=255)                    ggAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGtt                                             >  1:562728/1‑76 (MQ=255)                     gacaCCAGCGCCAGCCCCCCCCCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGttt                                            <  2:1021230/73‑1 (MQ=255)                     gAAACCAGCGCCAGCACCCCCCCTGTCCGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGttt                                            <  2:406144/76‑1 (MQ=255)                     gAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGttt                                            <  2:1497005/76‑1 (MQ=255)                       acccAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCa                                          <  1:1229114/74‑1 (MQ=255)                       aaCCAGGGCCAGCACCCCACATGTACGCTGATCGGTGACTACCAGCGCTGCGCGGCTACCCGCCAGCAGCGTTTCa                                          <  1:1391707/76‑1 (MQ=255)                        aCCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCaa                                         >  2:1113067/1‑76 (MQ=255)                        aCCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGAGGCTACCCGCCAGCAGCGTTTCaa                                         >  1:1451624/1‑76 (MQ=255)                           aGCGCCAGCACCCCCCCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAatat                                      <  1:654853/76‑1 (MQ=255)                            gcgcCAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAatata                                     <  2:914093/76‑1 (MQ=255)                               ccAGCCCCCCGCCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTg                                  <  1:363689/76‑1 (MQ=255)                               ccAGCACCCCACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTg                                  <  2:428308/76‑1 (MQ=255)                                  gCACCCCACCGGTCAGCGGAGAGGGGTCTACCAGAGCTGCGCGGCTACCCGCCCGCAGCGGTTCAATATATTGCTg                               <  2:223578/76‑1 (MQ=255)                                        ccccTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGCGttt                         <  2:1507899/74‑1 (MQ=255)                                        cACCTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGttt                         >  1:480070/1‑76 (MQ=255)                                           cTGTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTgag                      >  2:1012631/1‑76 (MQ=255)                                             gTACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGAt                    <  1:173867/76‑1 (MQ=255)                                              tACGCGGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAGTATATTGCTGGAGTTTGAGATc                   >  2:57113/1‑76 (MQ=255)                                                cgcgGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCa                 >  2:985319/1‑76 (MQ=255)                                                cgcgGATCGGTGACTACCAGAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCa                 <  2:985106/76‑1 (MQ=255)                                                        ggTGACTCNCTGAGGTGCGNGGCTACCCGCCAGCAGCGTTTCNATATATTGCTGGAGTTTGAGATCCAGCGTCAgg         <  2:1277220/76‑1 (MQ=255)                                                           gACTACCAGAGCGGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTaa      <  2:392441/76‑1 (MQ=255)                                                             cTACCAGAGCTGCGCGGCNACCCGCCAGCAGCGTTTCAAGATATTGGTGGAGTTTGAGATCCAGCGTCAGGTAAat    >  1:1464562/1‑76 (MQ=255)                                                               aCCAGAGCTGCGCGGCTCCCCGCCAGCAGCGTTTCAATATATCGCTGGAGTTTGAGATCCAGCGTCAGGTAAatat  <  2:1320245/76‑1 (MQ=255)                                                                   |                                                                        TTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCACCTGTACGCGGATCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATAT  >  REL606/649919‑650056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑