Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	118.3 / NA	31	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGG  >  REL606/1329443‑1329585                                                                          |                                                                      tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                     >  1:864341/1‑76 (MQ=255)        ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                              <  2:1529103/76‑1 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTANTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGANTTg                                                         >  2:1423091/1‑76 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                         >  2:1532712/1‑76 (MQ=255)              aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                        >  1:353114/1‑76 (MQ=255)               aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                       <  2:775210/76‑1 (MQ=255)                  ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAc                                                    <  2:847695/76‑1 (MQ=255)                  ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAc                                                    <  2:847684/76‑1 (MQ=255)                   cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                   >  2:757293/1‑76 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCGGAccg                                                  >  1:809002/1‑75 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                  <  1:1261727/76‑1 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                  <  1:513820/76‑1 (MQ=255)                     aaaCGATCAACAANTATCTGGGTAGTGACTGCGTGGTGAAATCCACCGTCGGTTACATCCGCGATTTGCCGACCAg                                                 <  2:1405680/76‑1 (MQ=255)                     aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                 <  1:643861/76‑1 (MQ=255)                     aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                 >  2:781368/1‑76 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                            >  2:1043146/1‑76 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                            >  1:625343/1‑76 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTAGTGAAAACCAGCGTCGGTTACATCCGCNATTTGCCGACCAGTGGCt                                            <  1:1460173/76‑1 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                           >  2:548126/1‑76 (MQ=255)                                aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCNGTGGCTCAGCTg                                      <  1:24204/76‑1 (MQ=255)                                aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                      >  2:549241/1‑76 (MQ=255)                                       tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                               >  1:1324216/1‑76 (MQ=255)                                       tGGGGNGTGACGACGTAGTGAAATCCAGCGTCGGTTANATCGTCGATTTGCCGNCCAGTGGCTCAGCTGCCaaaaa                               <  1:26360/76‑1 (MQ=255)                                         ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAaga                             <  1:208656/76‑1 (MQ=255)                                             gTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGc                         <  1:348022/76‑1 (MQ=255)                                              tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGcc                        >  1:754112/1‑76 (MQ=255)                                                 cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                     >  2:822351/1‑76 (MQ=255)                                                  tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                    >  2:362917/1‑76 (MQ=255)                                                  tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                    <  2:1241032/76‑1 (MQ=255)                                                        gtgAAATCCAGCGTCGGTTACATCCGCGATTTNCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTNCCt              >  2:1432804/1‑76 (MQ=255)                                                            aaTCCAGCGTCGGTNACATCCGCGATTTACCGACNAGTGGCTCNGCTGCCAAAAAGAGTGCCGCCTCTACCTccac          <  2:1262868/76‑1 (MQ=255)                                                              tCCAGCGTCGGTTGCATCCGCGATTTGCCGACCNGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca        >  2:1298891/1‑76 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa       >  1:930242/1‑76 (MQ=255)                                                                cggCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg      <  1:544881/74‑1 (MQ=255)                                                                  gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGAc    <  1:1219584/76‑1 (MQ=255)                                                                    gTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACgg  >  1:685870/1‑76 (MQ=255)                                                                          |                                                                      TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGG  >  REL606/1329443‑1329585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑