| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,156,704 | Δ1 bp | coding (1255/3093 nt) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,156,704 | 0 | C | . | 96.3% | 118.1 / ‑1.0 | 27 | coding (1255/3093 nt) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (13/13); minor base T (0/1); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGAT > REL606/3156636‑3156771 | cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGaa < 2:485781/76‑1 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGaaa > 1:221376/1‑76 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGaaaaa < 2:686281/76‑1 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGaaaaa < 2:1246688/76‑1 (MQ=255) ccGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTc < 2:574959/76‑1 (MQ=255) gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGC‑GTGGGAAAAAGTCTa > 2:807744/1‑76 (MQ=255) gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGGAAAAGTCTa > 2:807727/1‑76 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTAc < 2:1060870/76‑1 (MQ=255) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGt > 1:965057/1‑76 (MQ=255) cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCg < 2:453234/76‑1 (MQ=255) cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCg > 1:575409/1‑76 (MQ=255) cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCg < 2:1022966/76‑1 (MQ=255) aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAg > 2:1356049/1‑76 (MQ=255) cTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTc > 2:1249102/1‑76 (MQ=255) tttGCTAATTTCGGCGATATTAGCCGTATTACCGACGATCC‑TAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCg < 1:1105300/76‑1 (MQ=255) gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCa < 2:80910/76‑1 (MQ=255) aaTGTCGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCatat > 2:320550/1‑76 (MQ=255) cGGCGATATTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcg > 2:829522/1‑76 (MQ=255) gATATTAGGCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCa > 1:54253/1‑76 (MQ=255) atatTAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAg < 1:377083/76‑1 (MQ=255) ttAGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaa > 2:292097/1‑76 (MQ=255) aGCCGTATTACCGACGATCCG‑AGTGGGACAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaaac > 1:142555/1‑75 (MQ=255) aGCCGTATTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaaaa < 1:804615/76‑1 (MQ=255) aTTACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATcc > 2:1135436/1‑76 (MQ=255) tACCGACGATCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGt < 1:978573/76‑1 (MQ=255) cgacgaTCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGa > 2:226701/1‑76 (MQ=255) gacgaTCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGat < 1:987924/76‑1 (MQ=255) gacgaTCCG‑AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGat < 2:636334/76‑1 (MQ=255) | CGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGAT > REL606/3156636‑3156771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |