| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,846 | A→G | L487P (CTG→CCG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,846 | 0 | A | G | 97.3% | 149.2 / NA | 37 | L487P (CTG→CCG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/24); minor base T (1/0); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACT > REL606/473773‑473917 | aatCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCggg > 1:609071/1‑76 (MQ=255) atCTCAAGATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGa < 2:906009/76‑1 (MQ=255) tctcAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGAt > 1:603078/1‑76 (MQ=255) cAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAg > 2:986294/1‑76 (MQ=255) aaTATCCGGATTNTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGc > 1:19721/1‑76 (MQ=255) atatCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCTGGATCAGcc > 1:48729/1‑76 (MQ=255) atatCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGcc > 2:149929/1‑76 (MQ=255) tatCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATAAGCCa > 2:27068/1‑76 (MQ=255) ccGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCAttt < 1:876104/76‑1 (MQ=255) cGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCNTTGGGAATCGTCGGGATCAGCCAtttt < 1:1394417/76‑1 (MQ=255) cGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCAtttt < 2:150046/76‑1 (MQ=255) cGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCAtttt < 1:744462/76‑1 (MQ=255) ggATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTg < 2:839306/76‑1 (MQ=255) tttttCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGcac > 1:635717/1‑76 (MQ=255) ttttCGCGCGGGCAGATGCCACAATCTCGCCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGcaca < 1:1117724/76‑1 (MQ=255) tttCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACAt < 1:785230/76‑1 (MQ=255) ttCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACAtt > 1:643188/1‑76 (MQ=255) ttCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACAtt < 2:624057/76‑1 (MQ=255) cgcgcgGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTcc < 1:631682/76‑1 (MQ=255) cgcgGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAg < 1:1132072/76‑1 (MQ=255) cgcgGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAg < 2:1074118/76‑1 (MQ=255) gcgGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGa < 1:313125/76‑1 (MQ=255) cgGGCACATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCTTCGGGATCAGCCATTTTGCACATTCCAGAt < 1:1278726/76‑1 (MQ=255) gggCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATg > 1:639840/1‑76 (MQ=255) ggCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATgc < 2:440182/76‑1 (MQ=255) gCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATgcg > 2:254077/1‑76 (MQ=255) aGATGCCACAATCTNACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATgcgcn < 2:198645/76‑2 (MQ=255) ccACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTg < 1:409695/76‑1 (MQ=255) tctcCCCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAAt < 1:1038946/76‑1 (MQ=255) tcACCCGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAAttt < 2:745309/76‑1 (MQ=255) cACCCGCTTCATAACCGTTGGGAATCGTCGGCATCAGCCTNNTTGCACATTCNNGTTGCGCCAGTTGCTTAATttc > 1:1264934/1‑76 (MQ=255) cGCTTCATAACCGTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCttcg > 2:1355641/1‑76 (MQ=255) gTTGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTg < 2:972487/76‑1 (MQ=255) ttGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGc < 1:668799/76‑1 (MQ=255) ttGGGAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGc > 1:944025/1‑76 (MQ=255) gggAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGccc < 2:628264/76‑1 (MQ=255) ggAATCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTGTTCGTTCGCCGCATTGCCCa < 1:7615/76‑1 (MQ=255) aaTCGTCGGGATCAGCCATTTTGCACATTCCANATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCNAAt > 2:1301014/1‑76 (MQ=255) aTCGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATa < 1:244076/76‑1 (MQ=255) cGTCGGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACt > 1:963826/1‑76 (MQ=255) | AATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACT > REL606/473773‑473917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |