Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,164,745	A→C	D1297A (GAC→GCC)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,164,745	0	A	C	100.0%	165.8 / NA	41	D1297A (GAC→GCC)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/21);  total (20/21)

GAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGA  >  REL606/4164675‑4164817                                                                       |                                                                         gAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTNATGCCGTGa                                                                     <  1:31484/76‑1 (MQ=255) gAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGa                                                                     >  2:1129557/1‑76 (MQ=255)   aGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATNCTCACCTTTAAGTCTGATGCCGTGAAc                                                                   <  1:1400102/76‑1 (MQ=255)        gggNGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATNCTCACCGTTAAGTCTGATGCCGTGAACGGTCg                                                              <  2:1305088/76‑1 (MQ=255)         ggCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGt                                                             <  1:540705/76‑1 (MQ=255)          gcgcTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTa                                                            <  1:621032/76‑1 (MQ=255)          gcgcTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTa                                                            <  2:1396515/76‑1 (MQ=255)             cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg                                                              <  2:421514/71‑1 (MQ=255)             cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg                                                              >  1:421514/1‑71 (MQ=255)             cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg                                                              <  2:302618/71‑1 (MQ=255)             cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCg                                                              >  1:302618/1‑71 (MQ=255)             cTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCa                                                         >  1:144310/1‑76 (MQ=255)               ggAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGNCGTGAACGGTCGTACCAAg                                                       <  1:1302050/76‑1 (MQ=255)                 aaGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGAt                                                     >  2:952381/1‑76 (MQ=255)                  aGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCCTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATg                                                    >  2:1029111/1‑76 (MQ=255)                        cGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATaaa                                              <  2:15522/76‑1 (MQ=255)                          gcgcAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATaaaaa                                            <  1:1221651/76‑1 (MQ=255)                           cgcAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAAc                                           >  1:192140/1‑76 (MQ=255)                             cagcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACAt                                         >  1:779619/1‑76 (MQ=255)                             cagcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTCAACGGTCGTACCAAGATGTATAAAAACAt                                         >  2:376809/1‑76 (MQ=255)                              agcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATc                                        <  2:991008/76‑1 (MQ=255)                              agcaTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATc                                        <  1:1368301/76‑1 (MQ=255)                                 aTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTg                                     >  2:1372622/1‑76 (MQ=255)                                  tACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTgg                                    <  2:737524/76‑1 (MQ=255)                                    cacCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGAc                                  <  2:160336/76‑1 (MQ=255)                                     acCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACg                                 >  2:551281/1‑76 (MQ=255)                                     acCCTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACg                                 >  2:763171/1‑76 (MQ=255)                                      cccTGCAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACgg                                >  1:256999/1‑76 (MQ=255)                                           cAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc                           >  1:356376/1‑76 (MQ=255)                                           cAGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTAGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc                           <  1:871296/76‑1 (MQ=255)                                            aGGAAATGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcct                          >  1:304731/1‑75 (MQ=255)                                             ggAAATGCTCACCGTTAAGTCTGATTCCNTGAACGGTCCAAACAAGAGGTATAAAAACATCGTGGACGGCAACCAt                         <  1:122684/76‑1 (MQ=255)                                             ggAAACGCCCACCGTTAAGTCTGATCCCGTGAACGGTCGTACCAAGATGTATAAAACCACCGTGGACGGCAACCAt                         <  2:1030131/76‑1 (MQ=255)                                               aaaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCa                       >  1:383485/1‑76 (MQ=255)                                                aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg                      >  2:808035/1‑76 (MQ=255)                                                aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg                      <  1:969324/76‑1 (MQ=255)                                                aaTGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAg                      >  2:808037/1‑76 (MQ=255)                                                  tGCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGAt                    <  1:278979/76‑1 (MQ=255)                                                   gCTCACCGTTAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATg                   <  1:413928/76‑1 (MQ=255)                                                    cTCACCGTTAAGTCNGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATgn                  <  2:193693/76‑2 (MQ=255)                                                            tAAGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGc          >  2:561683/1‑76 (MQ=255)                                                             aaGTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCa         <  1:749770/76‑1 (MQ=255)                                                               gTCTGATGCCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATg       >  1:1061266/1‑76 (MQ=255)                                                                  tgatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCa    <  2:658360/76‑1 (MQ=255)                                                                  tgatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCa    >  2:291587/1‑76 (MQ=255)                                                                   gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCGTCAGATGGAGCCTGGCATGCCAg   >  2:1025348/1‑76 (MQ=255)                                                                   gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAg   <  1:835859/76‑1 (MQ=255)                                                                   gatgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAg   >  1:568506/1‑76 (MQ=255)                                                                    atgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGa  <  1:251925/76‑1 (MQ=255)                                                                    atgcCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGa  >  1:818225/1‑76 (MQ=255)                                                                       |                                                                         GAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGA  >  REL606/4164675‑4164817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑