Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	132.1 / NA	41	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/28);  total (13/28)

CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851                                                                        |                                                                         cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  1:536552/1‑76 (MQ=255)    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                   <  1:1345394/76‑1 (MQ=255)     gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATGAGCCGCCATAACAGCAAAACGCCAATCCccaaca                                                                  >  2:233341/1‑76 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                <  1:238891/76‑1 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                >  2:173932/1‑76 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGCGGAAATCAGCCCCAATAACAGCAAAACGCCCATCCCCACCAGa                                                                >  2:792540/1‑76 (MQ=255)         ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:726691/76‑1 (MQ=255)          cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  2:379394/76‑1 (MQ=255)           ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:448340/76‑1 (MQ=255)              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                         <  1:211539/76‑1 (MQ=255)                 cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      >  2:804831/1‑76 (MQ=255)                  aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     >  2:858456/1‑76 (MQ=255)                       cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:326014/76‑1 (MQ=255)                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:364470/76‑1 (MQ=255)                            ggTCTGGCTGTCGGAAATCAGCCCCCATAACCGCAAAACANNAATCCCCACCNNAGAAAGCATGACGCCTGTATTa                                           >  1:1256499/1‑76 (MQ=255)                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATNACGCCTGTATTa                                           <  1:21584/76‑1 (MQ=255)                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  2:437514/76‑1 (MQ=255)                             gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCNCCAGATAAAGCATGACGCCTGTATTaa                                          <  1:1395517/76‑1 (MQ=255)                             gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:761201/76‑1 (MQ=255)                                 ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:948079/76‑1 (MQ=255)                                 ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  2:1097889/1‑76 (MQ=255)                                    tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  1:508390/1‑76 (MQ=255)                                     gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:540139/76‑1 (MQ=255)                                      tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCNGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1178883/76‑1 (MQ=255)                                      tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1056552/76‑1 (MQ=255)                                      tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:401357/76‑1 (MQ=255)                                       cGGAAATCAGCCCCAATAACGGAAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:3057/1‑76 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:81923/76‑1 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:548622/76‑1 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  2:640603/1‑76 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  2:677080/1‑76 (MQ=255)                                           aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  2:473714/1‑76 (MQ=255)                                              cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:241116/76‑1 (MQ=255)                                                 ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:496269/1‑76 (MQ=255)                                                     aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  1:687076/76‑1 (MQ=255)                                                     aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  1:1087021/76‑1 (MQ=255)                                                     aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:993267/76‑1 (MQ=255)                                                     aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:531504/76‑1 (MQ=255)                                                      aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  2:693951/76‑1 (MQ=255)                                                        aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:1052679/1‑76 (MQ=255)                                                            gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  1:60553/76‑1 (MQ=255)                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:778205/76‑1 (MQ=255)                                                               aaaCNCCAATCCCCACCAGATAATGCATTACGGCTGTATNAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  1:173932/76‑1 (MQ=255)                                                                     cAACCCCCACAAAAAAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  <  1:666916/76‑1 (MQ=255)                                                                        |                                                                         CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑