Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,016,003	G→A	W475* (TGG→TAG)	ECB_02816 →	KpsD protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,016,003	0	G	A	100.0%	163.1 / NA	43	W475* (TGG→TAG)	ECB_02816	KpsD protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/19);  total (24/19)

GTCATCAATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTGGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACG  >  REL606/3015930‑3016071                                                                          |                                                                     gTCATCAANATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGc                                                                    <  1:1235103/76‑1 (MQ=255)      caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg                                                               >  2:954090/1‑76 (MQ=255)      caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg                                                               >  1:734654/1‑76 (MQ=255)      caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg                                                               <  2:618642/76‑1 (MQ=255)      caATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAg                                                               >  1:498625/1‑76 (MQ=255)           ttCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTAt                                                          >  2:404534/1‑76 (MQ=255)            tCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATg                                                         >  1:192754/1‑76 (MQ=255)             ccGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGa                                                        <  1:137805/76‑1 (MQ=255)              cGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGac                                                       >  1:99378/1‑76 (MQ=255)              cGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGac                                                       >  1:501082/1‑76 (MQ=255)                gagaAAACATCGCTGATTATGGTTCATGGGGAAGTGCTGTTCCCGAACGCTGTGAGCTAGCAGAAGGGTATGacca                                                     <  1:72910/76‑1 (MQ=255)                gagaAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGacca                                                     <  1:483662/76‑1 (MQ=255)                       cATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGa                                              <  1:790209/76‑1 (MQ=255)                         tCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGAtt                                            <  2:432489/76‑1 (MQ=255)                             tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt                                        >  1:256640/1‑76 (MQ=255)                             tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt                                        >  1:701653/1‑76 (MQ=255)                             tGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACAt                                        >  1:747862/1‑76 (MQ=255)                              gATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATc                                       <  2:498625/76‑1 (MQ=255)                              gATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATc                                       >  1:793359/1‑76 (MQ=255)                               aTTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCg                                      <  1:212161/76‑1 (MQ=255)                                  aTGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCgaga                                   >  2:665309/1‑76 (MQ=255)                                   tGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGaa                                  >  2:846161/1‑76 (MQ=255)                                    ggTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGCAGGGTATGACCACCGAGGATTAAATCGAGaaa                                 >  2:412709/1‑76 (MQ=255)                                     gTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAAt                                >  1:1106485/1‑76 (MQ=255)                                      ttCATGGCGAAGTGCTGTTCCCGAAAGCNGTGAGCTAGAAGGAGGGTGTGACCACCGAGGATTACATCGAGAAAtg                               <  1:125151/76‑1 (MQ=255)                                        cATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAAtgtg                             >  1:335853/1‑76 (MQ=255)                                         aTGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATgtgg                            <  2:1225900/76‑1 (MQ=255)                                            gCGAAGTGCTGTTCCCGAACGCGGTGAGCTATCAGAAGGANNTGACCACCGANNACTACATCGAGAAATGtggtgg                         >  1:1258131/1‑76 (MQ=255)                                              gAAGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGcc                       >  2:127783/1‑76 (MQ=255)                                               aaGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGATCACCGAGGATTACATCGAGAAATTTGGTGGCCt                      <  2:1194598/76‑1 (MQ=255)                                               aaGTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCt                      <  1:469089/76‑1 (MQ=255)                                                 gTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGa                    >  1:645010/1‑76 (MQ=255)                                                 gTGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGa                    >  1:488854/1‑76 (MQ=255)                                                  tGCTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGAc                   >  2:912828/1‑76 (MQ=255)                                                   gCTGTGCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACg                  <  2:1074956/76‑1 (MQ=255)                                                    cTGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGc                 <  1:888187/76‑1 (MQ=255)                                                     tGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGNCCTGACGCa                <  1:36878/76‑1 (MQ=255)                                                     tGTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCa                <  2:867887/76‑1 (MQ=255)                                                      gTTCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaa               >  1:708476/1‑76 (MQ=255)                                                       ttCCCGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaaa              >  2:298531/1‑76 (MQ=255)                                                         cccGAACGCGGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCaaaaa            >  2:836815/1‑76 (MQ=255)                                                                 cgGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTaa    >  2:519978/1‑76 (MQ=255)                                                                 cgGTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTaa    <  1:748278/76‑1 (MQ=255)                                                                   gTGAGCTAGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACg  <  1:1043067/76‑1 (MQ=255)                                                                          |                                                                     GTCATCAATATTCCGGAGAAAACATCGCTGATTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTGGCAGAAGGGTATGACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAAAAATCGGGTAACG  >  REL606/3015930‑3016071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑