| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,570,909 | T→A | I37N (ATC→AAC) | uspA → | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,570,909 | 0 | T | A | 97.8% | 168.5 / ‑3.6 | 46 | I37N (ATC→AAC) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (16/28); minor base G (0/1); total (16/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
CCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAAC > REL606/3570838‑3570980 | cccGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCAc < 1:524291/76‑1 (MQ=255) cGGAAAGCAAAGTTCTGGTAGAGAAAGCAATCTCTATGGCTCGCCCCCACAATGCGAAAGTTTCTCTGAACCACGt > 1:495288/1‑76 (MQ=255) gAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAg < 1:546908/76‑1 (MQ=255) aaaGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCCACAATGCGAAAGTTTCTCTGAACCACGTAGa > 2:344050/1‑76 (MQ=255) gCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGt < 1:986066/76‑1 (MQ=255) cAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAAAGCGAAAGTTTCTCTGAACCACGTAGATGTa > 1:914950/1‑76 (MQ=255) aaaGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTaa < 1:767119/76‑1 (MQ=255) aaaGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTaa < 1:242529/76‑1 (MQ=255) gTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCCACAATGCGAAAGTTTCTCTGAAACACGTAGATGTaaaat > 1:272650/1‑74 (MQ=255) tCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAactac < 1:340230/76‑1 (MQ=255) cTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCCACCATGCGAAAATTTCTCTGAACCACGTAGATGNAAactact > 1:1235777/1‑76 (MQ=255) ggTAGAGANAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTActct < 1:1234809/76‑1 (MQ=255) gTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTg < 2:504427/76‑1 (MQ=255) gTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTg < 1:591979/76‑1 (MQ=255) agagAAAGCAGTCTCTATGGCTCGCCCCTACCATGCGAAAATTTTTCTGATCCACGGAGATGTAAACTACTCTGAc > 1:1067404/1‑76 (MQ=255) gagaAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGAcc < 1:326524/76‑1 (MQ=255) gagaAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGAcc < 1:668094/76‑1 (MQ=255) agaAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCt < 1:847387/76‑1 (MQ=255) gaAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCta < 2:1052301/76‑1 (MQ=255) gaAAGCAGTATCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACCCTGACCta < 1:47995/76‑1 (MQ=255) aaaGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCtat < 1:749984/76‑1 (MQ=255) ctctATGGCTCGCCCCCACAATGCGAAAGTTTCTCTTAACCACGTAGATGTAAACTACTCTGACCTATACACCggg > 2:1010788/1‑76 (MQ=255) tctATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAGCCACGTAGATGTAAACTACTCTGACCTATACACCGGGc < 2:638153/76‑1 (MQ=255) tctATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGc > 2:13267/1‑76 (MQ=255) ctATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCt > 2:538043/1‑76 (MQ=255) ctATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCt > 2:1010390/1‑76 (MQ=255) aTGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTa < 2:569310/76‑1 (MQ=255) aTGGATCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAAGTACTCTGACCTATACACCTGGCNTa > 2:1318390/1‑76 (MQ=255) tGGCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTAt < 2:412208/76‑1 (MQ=255) gCTCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTg < 2:221674/76‑1 (MQ=255) gCTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATAAACCGGGCTTATTg > 1:994175/1‑76 (MQ=255) cTCGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGa > 2:1146007/1‑76 (MQ=255) cGCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAtg > 2:1133121/1‑76 (MQ=255) gCCCCTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAtgt < 2:550836/76‑1 (MQ=255) ccccTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAtgtg < 2:571603/76‑1 (MQ=255) cccTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGa < 2:375354/76‑1 (MQ=255) ccTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGaa > 1:854267/1‑76 (MQ=255) cTACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAAt > 1:796408/1‑76 (MQ=255) tACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATc < 2:568621/76‑1 (MQ=255) tACAATGCGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATc < 1:1088497/76‑1 (MQ=255) cAANGCACAAGTTTCTCTGAACCAGGTAGATGTAAACTACTCTGACCTATACATCGGGCTTATTGATGTGAATTTg < 2:1270147/76‑1 (MQ=255) cGAAAGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGa > 2:980723/1‑76 (MQ=255) aGTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATg > 2:435113/1‑76 (MQ=255) gTTTCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGc < 1:1157069/76‑1 (MQ=255) tttCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCa > 2:687562/1‑76 (MQ=255) ttCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAg > 1:987156/1‑76 (MQ=255) ttCTCTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAg < 1:778126/76‑1 (MQ=255) tctcTGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGa > 1:236069/1‑76 (MQ=255) tctGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGaaa < 1:198317/76‑1 (MQ=255) tctGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGaaa < 1:104899/76‑1 (MQ=255) ctGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAAc < 1:871354/76‑1 (MQ=255) ctGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAAc > 1:781617/1‑76 (MQ=255) ctGAACCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAAc > 1:549229/1‑76 (MQ=255) | CCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAAC > REL606/3570838‑3570980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |