| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 95.0% | 88.1 / ‑3.4 | 20 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (16/3); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGT > REL606/2131383‑2131524 | aaatgaTTTGTTTTTAAATGTCGAGATNACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:1291534/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:1068172/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:240262/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACACNATNAAAATTAATAGAGGNATGAAAGGCaaaa > 2:1166332/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:846730/76‑1 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa > 1:633451/1‑76 (MQ=255) gTTTTTAAATGTNGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:15112/1‑74 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCgaaaaaaacg > 1:40903/1‑74 (MQ=255) gTTTTTAAATGTCGACATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:534372/1‑74 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:1035246/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 2:469235/76‑1 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:292585/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:464249/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:12305/76‑1 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 2:737553/1‑76 (MQ=255) gATCACAAAAACGACAATATGAAAATAAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGca < 2:997181/76‑1 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGACCTCCCGGAGNGAAGCCGcaagag > 1:170956/1‑73 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcgc > 1:353826/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGcgcg > 1:735813/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGG‑NAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcgc > 2:1103683/1‑76 (MQ=255) aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg > 1:820854/1‑76 (MQ=255) tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt < 2:281916/76‑1 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 1:446142/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 1:343800/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:864241/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:635437/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTACGCAGGAt > 2:1234441/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:391044/1‑76 (MQ=255) gagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg > 1:262445/1‑76 (MQ=255) aaaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 1:92921/75‑1 (MQ=255) aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGGAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:1038363/1‑76 (MQ=39) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGT > REL606/2131383‑2131524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |