Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,437	C→A	G290V (GGG→GTG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,437	0	C	A	100.0%	119.8 / NA	32	G290V (GGG→GTG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/17);  total (15/17)

AAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGA  >  REL606/474364‑474505                                                                          |                                                                     aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACg                                                                    <  1:355291/76‑1 (MQ=255)     aGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACgg                                                                <  2:69930/76‑1 (MQ=255)        ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGgaga                                                             >  2:454331/1‑76 (MQ=255)        ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGgaga                                                             >  2:244812/1‑76 (MQ=255)        ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGgaga                                                             <  2:1011188/76‑1 (MQ=255)         taatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGAc                                                            >  2:1053295/1‑76 (MQ=255)             cGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACaaaa                                                        <  1:301509/76‑1 (MQ=255)              gCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACaaaaa                                                       >  2:463609/1‑76 (MQ=255)                           gCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCaaa                                          <  1:596212/76‑1 (MQ=255)                           gCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCaaa                                          <  1:596220/76‑1 (MQ=255)                             aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAAcg                                        <  2:755723/76‑1 (MQ=255)                                 ccAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTc                                    >  1:671270/1‑76 (MQ=255)                                  cAGNGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGNCGGAGACAAAAAACAGCACCGCAAACGCGTcg                                   <  2:1208423/76‑1 (MQ=255)                                  cAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                                   <  1:404128/76‑1 (MQ=255)                                  cAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                                   <  1:812651/76‑1 (MQ=255)                                      ggTTGCTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCa                               >  1:1156965/1‑76 (MQ=255)                                           cTGAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGc                          <  2:411516/76‑1 (MQ=255)                                             gAATCAGAATTAACGGATCNAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg                        >  1:1214213/1‑76 (MQ=255)                                             gAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg                        <  2:389734/76‑1 (MQ=255)                                             gAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg                        <  2:977732/76‑1 (MQ=255)                                             gAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg                        <  2:907461/76‑1 (MQ=255)                                             gAATCAGAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg                        >  2:128814/1‑76 (MQ=255)                                                   gAATTAACGGATCAAACAACATCACGACGTAGACAATAAACAGCACCGCAAACGCGTNGCGCAATGGCAGCGTATc                  <  1:1243520/76‑1 (MQ=255)                                                   gAATTAACGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATc                  <  1:1098067/76‑1 (MQ=255)                                                         aCGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggc            <  1:997801/76‑1 (MQ=255)                                                         aCGGATCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggc            >  1:742878/1‑76 (MQ=255)                                                            gATCAAACAACATCACGACGGAGACAAAAACCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTAggcggc         >  1:1182646/1‑76 (MQ=255)                                                             aTCAAACAACATCANGACGCAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggcggcn        <  2:178582/76‑2 (MQ=255)                                                             aTCAAACAACATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCa        >  2:826439/1‑76 (MQ=255)                                                               cAAACAACATCACGACGGACACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACg      >  1:411917/1‑76 (MQ=255)                                                                 aacaacATCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACggg    >  1:382146/1‑75 (MQ=255)                                                                  acaacaTCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg   >  2:245893/1‑76 (MQ=255)                                                                  acaacaTCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGGATCGTGGGCGGCGCGGTg   >  2:1270312/1‑76 (MQ=255)                                                                   caacaTCACGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGa  <  1:804868/76‑1 (MQ=255)                                                                          |                                                                     AAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGA  >  REL606/474364‑474505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑