| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,382 | T→G | T60P (ACC→CCC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,382 | 0 | T | G | 97.5% | 152.1 / ‑3.7 | 40 | T60P (ACC→CCC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (21/18); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
ATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCA > REL606/4202309‑4202454 | aTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGATTAGCAGGCGGTGGGGCg > 1:1224241/1‑76 (MQ=255)aTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTCCGGTTAGCAGGCGGGGGGGCg > 1:383224/1‑76 (MQ=255) ttGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGt < 1:569912/76‑1 (MQ=255) tGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTg < 1:1057563/76‑1 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCACGCAACCCTGCTGTTGCATTGTGGGTAGCAGGCGGGGGGGCGTGGAAt > 1:484063/1‑76 (MQ=255) aaTGTCCCAGTTCNCCGACCTGACGCACNAANCCCTGCTGTTGCATTGGGGTTAGCAGGCGGTGGGGCGTGGAAtt < 2:1168647/76‑1 (MQ=255) aaTGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAtt < 2:299542/76‑1 (MQ=255) tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTgg > 2:852630/1‑76 (MQ=255) tCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGt < 1:1022444/76‑1 (MQ=255) cccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTa > 1:597287/1‑76 (MQ=255) aGTTCGCCGACCTGACGCACGAACCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAAcc < 1:1021946/76‑1 (MQ=255) tCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCgg > 1:124619/1‑76 (MQ=255) tCGCCGACCCGACGCACGAAACCCTGCTGTTGCCTTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCgg > 2:1115655/1‑76 (MQ=255) cGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGc < 2:967749/76‑1 (MQ=255) cGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGc < 1:1155425/76‑1 (MQ=255) ccGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCtt < 1:973738/76‑1 (MQ=255) cTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgc > 1:653248/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcg > 1:297366/1‑76 (MQ=255) gACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcgc > 2:447235/1‑76 (MQ=255) cGCACGAAACCTTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCa < 1:197718/76‑1 (MQ=255) cACGAAACCCTGCTGTTGCATTGTGGTTAGCCGGCGGGGGGGCGTGGAATTGGGAAACCCGGCTTGTTGCACCAGt > 2:850184/1‑76 (MQ=255) gAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTcc < 2:508653/76‑1 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg < 2:905381/76‑1 (MQ=255) aCCCGGTTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGGAACCCGGCTTGTTGCGCCAGTTCCGTg < 1:1277864/76‑1 (MQ=255) gctgTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGc > 2:1200419/1‑76 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCca > 2:454787/1‑76 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGCGGGTGCca > 1:191498/1‑76 (MQ=255) gTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcac > 2:83781/1‑76 (MQ=255) ttGCATTGTGGTTAGCAGGCGGTGGGGNGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcaca > 1:1289582/1‑76 (MQ=255) tGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCacac < 2:1197566/76‑1 (MQ=255) gCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACt > 2:267547/1‑76 (MQ=255) ttGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGACAGTTCCGTGNGTGCCACACtact > 1:163719/1‑73 (MQ=255) tgtgGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCa > 1:348862/1‑76 (MQ=255) ggTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTg > 1:467271/1‑76 (MQ=255) tAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGGCACACTGCCAtt > 1:432653/1‑72 (MQ=255) tAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt < 2:432653/72‑1 (MQ=255) tAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAt < 1:126270/76‑1 (MQ=255) aGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGTGCCAGTTCCGTGAGTGCCACACTGCCATTGGAtt < 1:817939/76‑1 (MQ=255) gcgggCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTc < 1:1078932/73‑1 (MQ=255) gCAGGCGGTGGGGCGTGGAATTGGGTAACCCCGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTc < 2:209416/76‑1 (MQ=255) aGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 2:1051521/1‑76 (MQ=255) cGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCaa > 1:618184/1‑76 (MQ=255) ggTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAAt > 2:534106/1‑76 (MQ=255) ggggCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCa < 2:422055/76‑1 (MQ=255) | ATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCA > REL606/4202309‑4202454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |