Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,404,024	T→C	C11R (TGC→CGC)	rpsR →	30S ribosomal protein S18

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,404,024	0	T	C	100.0%	209.3 / NA	51	C11R (TGC→CGC)	rpsR	30S ribosomal protein S18
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)

GCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCG  >  REL606/4403959‑4404096                                                                  |                                                                         gCAGATTGAATTGATAGATNCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCAc                                                                >  1:1218081/1‑76 (MQ=255)  cAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCAcc                                                               <  2:887248/76‑1 (MQ=255)   aGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACcg                                                              >  2:522567/1‑76 (MQ=255)   aGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACcg                                                              >  2:51841/1‑76 (MQ=255)     attgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACcgcg                                                            <  2:820764/76‑1 (MQ=255)       tgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGa                                                          <  2:1153176/76‑1 (MQ=255)       tgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGa                                                          >  2:749734/1‑76 (MQ=255)         aattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAACTTCCGCCGTTTCACCGCGGAAg                                                        >  1:355570/1‑76 (MQ=255)           ttgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGc                                                      >  1:466779/1‑76 (MQ=255)              aTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGtt                                                   <  2:947003/76‑1 (MQ=255)                    tCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAagag                                             <  1:559210/76‑1 (MQ=255)                    tCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAagag                                             <  2:345047/76‑1 (MQ=255)                      tGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                           <  2:840389/76‑1 (MQ=255)                        gagaTTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                         <  2:895/76‑1 (MQ=255)                        gagaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                         <  2:722259/76‑1 (MQ=255)                        gagaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                         <  2:233787/76‑1 (MQ=255)                         agaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTTACCGCGGAGGGCGTTCAAGAGATCgc                                        >  2:374813/1‑75 (MQ=255)                         agaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                        >  2:1029043/1‑76 (MQ=255)                             tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtat                                    <  1:213958/76‑1 (MQ=255)                               gCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATaa                                  <  1:456688/76‑1 (MQ=255)                                 cATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAg                                <  2:459323/76‑1 (MQ=255)                                 cATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAg                                >  2:222493/1‑76 (MQ=255)                                 cATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAg                                <  1:83363/76‑1 (MQ=255)                                 cATATGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGACATCGACTATAAAg                                <  1:83383/76‑1 (MQ=255)                                  atatGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGa                               >  2:576458/1‑76 (MQ=255)                                    atGGCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGata                             >  2:967428/1‑76 (MQ=255)                                       gCACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                          <  1:544235/76‑1 (MQ=255)                                        cACGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGc                         >  2:419966/1‑76 (MQ=255)                                         aCGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCt                        <  2:1015171/76‑1 (MQ=255)                                         aCGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCt                        <  2:793470/76‑1 (MQ=255)                                          cGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTa                       <  1:753448/76‑1 (MQ=255)                                          cGTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTa                       <  2:902318/76‑1 (MQ=255)                                           gTTATTTCCGTCGTCGCAAGTTCCGCCGTTTCATCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTAc                      >  1:213121/1‑76 (MQ=255)                                            ttATTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACg                     >  2:560683/1‑76 (MQ=255)                                              aTTTCCGTCGTCGCAAGTTCCGCCGTTTCACCGCGGTAGGCGTTCAAGAGATCGACTATAAANATATCGCTACCCt                   >  1:162449/1‑76 (MQ=255)                                                  ccGTNGTCGCAAGTTCCGCCGTTGCACCCCGTTAGGCGTNCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa               <  1:153981/76‑1 (MQ=255)                                                  ccGTCGTCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa               <  1:638085/76‑1 (MQ=255)                                                   cgtcgtCGCAAGTTCCGCCGTTTGACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaa              <  1:1111206/76‑1 (MQ=255)                                                   cgtcgtCGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaa              <  1:572201/76‑1 (MQ=255)                                                    gtcgtcGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaaa             >  1:217223/1‑76 (MQ=255)                                                    gtcgtcGCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaaa             >  1:360579/1‑76 (MQ=255)                                                     tcgtcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAAc            >  2:330807/1‑76 (MQ=255)                                                      cgtcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGGCTATAAAGATATCGCTACGCTGAAAAACt           <  1:466678/76‑1 (MQ=255)                                                      cgtcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTNCGCTGAAAAACt           <  1:20207/76‑1 (MQ=255)                                                      cgtcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt           >  2:50465/1‑76 (MQ=255)                                                      cgtcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt           >  2:387765/1‑76 (MQ=255)                                                        tcgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc         <  2:613524/76‑1 (MQ=255)                                                        tcgCAAGTCCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc         <  2:613544/76‑1 (MQ=255)                                                         cgCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACa        >  1:627567/1‑76 (MQ=255)                                                          gCAAGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACAt       >  1:553814/1‑76 (MQ=255)                                                             aGTTCCGCCGTTTCACCGCGGAAGGCGTTCAAGNGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCAc    >  2:1105529/1‑76 (MQ=255)                                                               ttCCGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCg  <  1:122757/76‑1 (MQ=255)                                                                  |                                                                         GCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCG  >  REL606/4403959‑4404096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑