Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT100.0% 131.7 / NA 39L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/18);  total (21/18)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851
                                                                        |                                                                        
ccGATGCATCCGGCGTCCCGGAGCAGCTGGTCTGGCTGTCGGAAATCAGACCCAATAACAGCAAAACGCCAATccc                                                                       <  1:1221618/76‑1 (MQ=255)
 cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  2:440571/1‑76 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     >  1:212280/1‑76 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCAGATGGAAATCAGNCCACATAACAGCAAAACGCCAATCCcca                                                                     >  1:120590/1‑76 (MQ=255)
      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:1197306/76‑1 (MQ=255)
       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                >  1:702473/1‑76 (MQ=255)
       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGTGGAAAATAGACCCAATAACAGGAAAACGCCAATACCCACCAGa                                                                >  1:700816/1‑76 (MQ=255)
        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  1:55198/76‑1 (MQ=255)
        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  1:222527/76‑1 (MQ=255)
           ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:897729/76‑1 (MQ=255)
             cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:832149/76‑1 (MQ=255)
              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                         <  2:467033/76‑1 (MQ=255)
                   ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    <  1:888957/76‑1 (MQ=255)
                    gcgcGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCAACAGATAAAGCATGACGc                                                   <  1:199095/76‑1 (MQ=255)
                       cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:497958/76‑1 (MQ=255)
                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:938262/76‑1 (MQ=255)
                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  1:821419/1‑76 (MQ=255)
                           tGGTCTGGCTGTCGGAACTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:138890/76‑1 (MQ=255)
                           tGGTCTGGCTGTCGGAAATCAGCNCCAATNACAGCTAAACGCCANTCTCCAACAGATAAAGTATGACGCCTGTAtt                                            >  2:163281/1‑76 (MQ=255)
                           tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:342620/1‑76 (MQ=255)
                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:238378/1‑76 (MQ=255)
                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:692177/76‑1 (MQ=255)
                              tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:683902/1‑76 (MQ=255)
                              tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:377129/76‑1 (MQ=255)
                               cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:1069341/1‑76 (MQ=255)
                                  gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAATGCATGACGCCTGTATTAAACAgg                                     >  1:1111721/1‑76 (MQ=255)
                                   cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  2:120590/76‑1 (MQ=255)
                                    tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:794170/76‑1 (MQ=255)
                                     gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:1157562/1‑76 (MQ=255)
                                      tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:857010/76‑1 (MQ=255)
                                          aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:384977/1‑76 (MQ=255)
                                                 ccccAATAACAGCAAAACGCCAAGCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:743487/76‑1 (MQ=255)
                                                   ccAATAACAGCAAAACGACAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:429264/1‑76 (MQ=255)
                                                      aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:854341/1‑76 (MQ=255)
                                                      aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:1100771/1‑76 (MQ=255)
                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  1:56357/1‑76 (MQ=255)
                                                          cAGCGAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  2:1283567/1‑76 (MQ=255)
                                                              aaaaCGCCAATCTCCACCAGATNANGCTTCACGCCNGTATTAAACAGGCTNCTTCCCGATTGCGCATTGCTCActa         <  2:22481/76‑3 (MQ=255)
                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:641625/1‑76 (MQ=255)
                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:851143/1‑76 (MQ=255)
                                                               aaaCGCCAATCCCCACCAGATAAAGCATGGCGCCTGCATTAAACAGGCTTTTTACCGNTGGCGCATCGCGCACCAg        <  1:1241477/76‑1 (MQ=255)
                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1123915/1‑76 (MQ=255)
                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:613455/1‑76 (MQ=255)
                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:588534/1‑76 (MQ=255)
                                                                        |                                                                        
CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑