| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,705,603 | G→A | intergenic (+65/‑26) | ygaH → / → emrR | predicted inner membrane protein/DNA‑binding transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,705,603 | 0 | G | A | 93.3% | 151.8 / 0.3 | 45 | intergenic (+65/‑26) | ygaH/emrR | predicted inner membrane protein/DNA‑binding transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (24/18); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGC > REL606/2705530‑2705673 | aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACt > 1:1073107/1‑76 (MQ=255) atataACCCACAAGAATCATTTTTCTACAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGAGTGaa > 1:1288698/1‑76 (MQ=255) tataACCCCCAAGAATCATTTTTCTAAAACAATACATTGACTTTGTTTTTCACTGTCGTTACTAGATAGACTGcaa > 1:1178537/1‑76 (MQ=255) aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTaa < 2:671261/76‑1 (MQ=255) ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATg < 2:742708/76‑1 (MQ=255) cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGa < 2:1018726/76‑1 (MQ=255) acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAg < 2:214812/76‑1 (MQ=255) aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTc < 1:487767/76‑1 (MQ=255) gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCa < 2:248839/76‑1 (MQ=255) gAATCATTTTTCTAAAACAATACATTTACTTTATTTGACACTGTCGTTACTATATCGACTGAAATTAATGAGGTCa > 2:1289763/1‑76 (MQ=255) tCATATTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATAc > 1:80569/1‑76 (MQ=255) aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATAccc > 1:291461/1‑76 (MQ=255) tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCa < 2:1028764/76‑1 (MQ=255) tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCa > 1:332591/1‑76 (MQ=255) tttttCTAAAACAATACANTTACTTTATTTGTCACTGTCCTTACTAGATCGACTGAAATTAATGAGGTCATACCCa > 1:1241785/1‑76 (MQ=255) ntttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAANGAGGTCATACCCaa > 2:184632/2‑76 (MQ=255) tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCaaa > 1:181533/1‑76 (MQ=255) cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATgg > 1:538776/1‑76 (MQ=255) tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGa > 1:527500/1‑76 (MQ=255) aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACTCAAATGGATa < 1:510235/76‑1 (MQ=255) aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATa > 1:1003686/1‑76 (MQ=255) aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATa < 1:510250/76‑1 (MQ=255) aaCACTACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAg < 1:1284688/76‑1 (MQ=255) aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAg < 1:537168/76‑1 (MQ=255) tACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGt < 1:987729/76‑1 (MQ=255) tttACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTAc > 1:928858/1‑76 (MQ=255) ttACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACg > 1:831821/1‑76 (MQ=255) aCTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGcc > 1:585683/1‑76 (MQ=255) aCTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGcc > 1:281472/1‑76 (MQ=255) cTTTATTTGTCACTGACGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGccc < 2:37816/76‑1 (MQ=255) ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt > 2:1054564/1‑76 (MQ=255) ttATTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt > 1:1049734/1‑76 (MQ=255) aTTTGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg > 2:799317/1‑76 (MQ=255) tttGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa > 1:478278/1‑76 (MQ=255) tttGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa < 2:1291646/76‑1 (MQ=255) tGTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc > 1:597619/1‑76 (MQ=255) gTCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACANAANTGGATAGTTCGTTTNCGCCCATTGAACa > 2:1174809/1‑76 (MQ=255) tCACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa > 1:725211/1‑76 (MQ=255) cACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa < 2:245402/76‑1 (MQ=255) cACTGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa < 2:245403/76‑1 (MQ=255) tGTCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc > 1:997039/1‑76 (MQ=255) gTCGNTACTATATCGACTGAAATGAATGAGGTCATACCCNAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt < 1:161244/76‑1 (MQ=255) tCGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa > 1:579163/1‑76 (MQ=255) cGTTACTATATCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaa > 1:1076496/1‑76 (MQ=255) gTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa > 1:839979/1‑76 (MQ=255) gTTACTATATCGACTGAAANTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa > 1:1216578/1‑76 (MQ=255) tataTCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc < 2:648806/76‑1 (MQ=255) tatCGACTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc < 1:1015488/76‑1 (MQ=255) | ATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGC > REL606/2705530‑2705673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |