| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,991 | (T)5→4 | intergenic (‑192/‑131) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,987 | 0 | T | . | 91.1% | 175.3 / 14.0 | 45 | intergenic (‑188/‑135) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base . (16/25); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGT > REL606/3911914‑3912056 | cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACtattttattt > 2:1110651/1‑76 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTattttatttt < 1:396151/76‑1 (MQ=255) gACAAAGGATGAACTACCAGGAAGGGAACAACATTCATACTGAAATTGAA‑ATTTTTTCACTCACTatttaattnta > 2:1143756/1‑75 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTAaaaa < 2:217326/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaaaaac > 2:1042878/1‑75 (MQ=255) tGANCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTNTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 2:1124806/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa > 2:754846/1‑76 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAtt < 1:658957/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta < 1:1189202/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta < 2:136871/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTta > 1:994655/1‑76 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtat < 1:229790/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTatat > 2:1068798/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGaa < 2:86272/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatt < 2:309763/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatta < 2:267112/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatta < 2:18024/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattat < 1:262247/76‑1 (MQ=255) caacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAattatt > 1:436117/1‑76 (MQ=255) acaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaa > 1:705676/1‑76 (MQ=255) caTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:415802/76‑1 (MQ=255) cATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 2:148404/1‑76 (MQ=255) cATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca < 2:325406/76‑1 (MQ=255) aTACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat < 1:214607/76‑1 (MQ=255) tACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 1:506170/1‑76 (MQ=255) aCTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca > 1:488443/1‑76 (MQ=255) gtcaaaTTGAA‑TTNTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:156759/73‑1 (MQ=255) tGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 1:559936/76‑1 (MQ=255) tGAAATTGAA‑TTTTTGTCACTCTCTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:36188/76‑1 (MQ=255) aaaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGTAAGTATTAAACGCATCATaaa > 1:1133304/1‑76 (MQ=255) aaaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:270070/76‑1 (MQ=255) aaaTTGAA‑NTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:1064312/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:962073/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:725032/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:607879/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:544640/76‑1 (MQ=255) aTTGAA‑TTNTATTCACTCANTATTTTATTTTTAAAAAACAACNATTTATATTGAAATTATTAAACGCATCATaaaa < 2:1002840/76‑1 (MQ=255) ttGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAGAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt < 2:130120/76‑1 (MQ=255) tGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc > 1:250765/1‑76 (MQ=255) ttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa < 2:253780/76‑1 (MQ=255) tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa > 1:845388/1‑76 (MQ=255) tcactcacTATTTTA‑TTTTATAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaaa > 1:2983/1‑76 (MQ=255) tcactcacTATTTTA‑TTTTAAAAATCAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaaa > 2:328798/1‑76 (MQ=255) ctcactATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata < 1:240795/76‑1 (MQ=255) tcactATTTTA‑TTTTAAAAAGCCACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATc > 1:643/1‑76 (MQ=255) tcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATc > 1:94637/1‑76 (MQ=255) actATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 2:678658/1‑76 (MQ=255) ctATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg < 2:529789/76‑1 (MQ=255) tattttatttTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg < 1:968767/76‑1 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt > 2:1206592/1‑76 (MQ=255) | CGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGT > REL606/3911914‑3912056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |