Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	798,273	(A)7→6	coding (1003/1146 nt)	ECB_00739 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	798,267	0	A	.	93.5%	126.9 / 4.8	31	coding (1009/1146 nt)	ECB_00739	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (15/14);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACCTATATTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGAAAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACAAAGCAA  >  REL606/798193‑798338                                                                           |                                                                        aCCTATATTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaa                                                                        <  1:171270/76‑1 (MQ=255) aCCTATATTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaa                                                                        <  1:171286/76‑1 (MQ=255) aCCTATATTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaa                                                                        <  2:458190/76‑1 (MQ=255)    tataTTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaa                                                                     >  1:414006/1‑76 (MQ=255)    tataTTTATCATTGGCTTTTCAATGAGAATACATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaa                                                                     <  2:199664/76‑1 (MQ=255)       atTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaaaca                                                                  <  1:954439/76‑3 (MQ=255)       atTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaaaca                                                                  <  1:954427/76‑3 (MQ=255)       atTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaaaca                                                                  <  1:954421/76‑3 (MQ=255)        tttATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGaaaaaacac                                                                 <  1:703303/76‑4 (MQ=255)           aTCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGa                                                             <  1:579798/76‑1 (MQ=255)             cATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGAAAAAAACACAGaa                                                            <  1:972162/76‑1 (MQ=255)             cATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGaaa                                                           >  1:649020/1‑76 (MQ=255)                 ggCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATcc                                                       >  1:416951/1‑76 (MQ=255)                          aTGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTAt                                              >  1:490359/1‑76 (MQ=255)                             agaATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGcc                                           >  1:1042790/1‑76 (MQ=255)                                 tatatatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAAtt                                       <  2:507677/76‑1 (MQ=255)                                  atatatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCANTTATGCCAAttt                                      <  1:13448/76‑1 (MQ=255)                                  atatatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAAttt                                      >  1:518356/1‑76 (MQ=255)                                  atatatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAAttt                                      >  1:515011/1‑76 (MQ=255)                                  atatatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAAttt                                      >  2:829568/1‑76 (MQ=255)                                    atatTGGATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCa                                    <  1:235592/76‑1 (MQ=255)                                         ggATATGATGCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAg                               >  2:816626/1‑76 (MQ=255)                                               gatgCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGc                         <  2:959711/76‑1 (MQ=255)                                                atgCAGTTATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGcc                        >  2:563099/1‑76 (MQ=255)                                                  gCNGTTGTTGAGATAATAACCAAGG‑GAAAAACACAGAAAATCCACCAGTTATGCCAATATCAGCAAGGTCTTCCtt                      <  2:1139692/76‑1 (MQ=255)                                                    aGTTATTGAGATAATAACCAAGG‑AAAANACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTtat                    >  1:1206256/1‑76 (MQ=255)                                                      ttATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTataa                  <  2:134779/76‑1 (MQ=255)                                                       tATTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTataat                 <  2:447608/76‑1 (MQ=255)                                                        aTTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTataata                >  2:922304/1‑76 (MQ=255)                                                        aTTGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTataata                >  2:215114/1‑76 (MQ=255)                                                          tGAGATAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATaga              >  2:318570/1‑76 (MQ=255)                                                            agaTAATAACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATagaag            <  1:204407/76‑1 (MQ=255)                                                              ataataACCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAgaagaa          <  2:1149475/76‑1 (MQ=255)                                                               taataaCCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAAc         <  2:16756/76‑1 (MQ=255)                                                                aataaCCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACa        >  1:252524/1‑76 (MQ=255)                                                                 ataaCCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACaa       <  1:860579/76‑1 (MQ=255)                                                                  taaCCAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGNAGAACaaa      >  1:1054046/1‑76 (MQ=255)                                                                   aaCCAAGGAAAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACaaa      >  2:533631/1‑76 (MQ=255)                                                                     ccAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACAAAGCa   <  1:807187/76‑1 (MQ=255)                                                                      cAAGG‑AAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACAAAGCaa  <  2:304397/76‑1 (MQ=255)                                                                           |                                                                        ACCTATATTTATCATTGGCTTTTCAATGAGAATATATATTGGATATGATGCAGTTATTGAGATAATAACCAAGGAAAAAAACACAGAAAATCCACCAGTTATGCCAATTTCAGCAAGGTCTGCCTTATAATAGAAGAACAAAGCAA  >  REL606/798193‑798338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑