| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 21.9% | 172.2 / 30.3 | 64 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (32/18); new base T (14/0); total (46/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAG > REL606/1747828‑1747976 | cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGAAGAACGCTTATGAACTCCAgg > 2:510919/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:767802/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:402303/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCAc > 2:28261/1‑76 (MQ=255) cTGATAATGTTGTTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:956050/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:300574/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:712907/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAca > 1:893537/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:654245/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:413946/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 2:768319/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 1:103077/1‑76 (MQ=255) tGATAATGTTATTAATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGTACTCCAGTCACtt > 1:78255/1‑75 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcctt > 1:268863/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGcc > 1:631187/1‑76 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTcc < 1:1181581/76‑1 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAAATCCAGTCAcatc > 1:348130/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:264763/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGGACTCCg < 1:40475/76‑1 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGccaa > 1:735513/1‑74 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc < 1:189981/76‑1 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTACAGTCACGccaat > 1:1032380/1‑73 (MQ=255) gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCata < 1:290088/76‑1 (MQ=255) gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCata > 2:341292/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 1:1168220/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 2:571981/1‑76 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 1:539984/1‑76 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 1:539973/1‑76 (MQ=255) ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttttt > 2:212094/1‑76 (MQ=255) atTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCa > 1:328483/1‑76 (MQ=255) ttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt > 2:569047/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg > 2:896420/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTNTTGCCATg > 1:1059848/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTNTTGCCATg > 1:1059861/1‑76 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa < 1:529307/76‑1 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:539610/1‑76 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 2:555024/1‑76 (MQ=255) tATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc > 2:1076595/1‑76 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa > 2:969395/1‑76 (MQ=255) tGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt < 1:280191/76‑1 (MQ=255) gTTTTTTTCATGCAGCCATTACACGATGAACGCTTATAACCTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt < 1:297432/76‑1 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt < 2:626286/76‑1 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt < 2:1119241/76‑1 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 2:115771/1‑76 (MQ=255) ttATTTCATGCAGCCATTGCATGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga < 1:1140302/76‑1 (MQ=255) ttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 2:1112779/1‑76 (MQ=255) ttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 1:442197/1‑76 (MQ=255) ctgtttCATGCAGNCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga < 1:162995/73‑1 (MQ=255) ctctttCATGCAGNCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga < 1:162985/73‑1 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATGTTATTGCCATGACTATtgatg > 1:1133363/1‑76 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTCCCATGACTATtgatg > 2:40576/1‑76 (MQ=255) ttCATCCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa < 1:689689/76‑1 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg > 1:572112/1‑76 (MQ=255) aTGCAGCCATTACACGATGACCGTTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc < 1:743289/76‑1 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 2:267114/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 1:977134/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACCCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 1:1073678/1‑76 (MQ=255) aGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGa < 2:59758/76‑1 (MQ=255) ccATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAcc > 1:491444/1‑76 (MQ=255) cATTACACGACGAACGCTTATGAACTCCAGGCACTCCGCCGTATTTTTGCCATGACTATTGATGAATGCGCGGCCt < 2:597609/76‑1 (MQ=255) tACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa < 2:54208/76‑1 (MQ=255) acacGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt > 1:975320/1‑76 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 2:210222/76‑1 (MQ=255) agCTTATGTACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATCGCCCAGAcc > 1:39759/2‑76 (MQ=255) cTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg > 1:381148/1‑76 (MQ=255) cTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg > 2:98205/1‑76 (MQ=255) ttATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt < 2:1168220/76‑1 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa < 2:312208/76‑1 (MQ=255) aGGCACTCNGCCATATTTTNGCCATGACTATTGATGAATGCGNGACCTGGATTGCCCAGACCGGTGACAGTGAAAg < 2:1007050/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAG > REL606/1747828‑1747976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |