Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,251,892	G→A	P290L (CCG→CTG)	nusA ←	transcription elongation factor NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,251,892	0	G	A	100.0%	128.0 / NA	34	P290L (CCG→CTG)	nusA	transcription elongation factor NusA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

CAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCA  >  REL606/3251819‑3251964                                                                          |                                                                         cAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGt                                                                        >  1:258236/1‑76 (MQ=255)     ttACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCa                                                                    >  1:181329/1‑76 (MQ=255)     ttACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCa                                                                    <  2:640852/76‑1 (MQ=255)       aCCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCAtt                                                                  <  2:776114/76‑1 (MQ=255)         cGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGc                                                                <  2:13502/76‑1 (MQ=255)             ttCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTa                                                            <  1:191762/76‑1 (MQ=255)               cAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGNCATTGCGTTAAt                                                          <  1:12990/76‑1 (MQ=255)               cAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAAt                                                          >  2:440947/1‑76 (MQ=255)                 aCGNCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGNAGCAACGTCTGCCAGTGCCATTGCGTTAATCa                                                        <  2:1127242/76‑1 (MQ=255)                  nggCGATATCCCTGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCNATTGCGTTAATCAc                                                       >  2:174431/2‑76 (MQ=255)                    gCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGa                                                     <  2:365980/76‑1 (MQ=255)                       atatCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACt                                                  <  2:106164/76‑1 (MQ=255)                            cATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGcc                                             >  1:507494/1‑76 (MQ=255)                             aTGGTGTGTTTATGTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCg                                            >  1:84909/1‑76 (MQ=255)                               ggTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCggg                                          <  2:599001/76‑1 (MQ=255)                                 tgtgtTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGtt                                        <  2:908363/76‑1 (MQ=255)                                 tgtgtTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGtt                                        <  1:1104964/76‑1 (MQ=255)                                          cTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCca                               <  2:945873/76‑1 (MQ=255)                                            tCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCcaca                             <  1:830782/76‑1 (MQ=255)                                                ccaccaCGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAc                         <  1:428385/76‑1 (MQ=255)                                                ccaccaCGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAc                         >  1:260852/1‑76 (MQ=255)                                                 caccacGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACg                        <  1:849408/76‑1 (MQ=255)                                                   ccacGTTAGAAGCTACGTGGGCCAGTTCCAGTGCGTTAATCTCGAACTGCGCCGGGTTTTCATCCCACAGGACGat                      <  2:919135/76‑1 (MQ=255)                                                    cacGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGata                     >  2:779319/1‑76 (MQ=255)                                                       gATAGAAGCAACGTCTGCCAGTGCCTTTGCGCTAATCACANNCTGCGCCGGGNNACCATCCCACAGGACGATATCg                  >  1:1084009/1‑76 (MQ=255)                                                       gATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCg                  >  2:10918/1‑76 (MQ=255)                                                          aGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATa               >  2:1176549/1‑76 (MQ=255)                                                          aGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACCGCGCCGGGTTATCATCCCACAGGACGATATCGATa               <  1:504193/76‑1 (MQ=255)                                                            aaGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACg             <  2:832596/76‑1 (MQ=255)                                                               cAACGTCTGCCAGTGCCATTGCGTTAATCACTAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTc          <  2:1181213/76‑1 (MQ=255)                                                               cAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTc          <  2:491186/76‑1 (MQ=255)                                                                aaCGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCg         <  1:926066/76‑1 (MQ=255)                                                                aaCGGCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCGCAGGACGATATCGATACGCTCg         <  1:926048/76‑1 (MQ=255)                                                                   gTCTGCCAGAGCCATTGCGTTAANCACGANCTGCGGCGGGTTATNATACCACAGGACGATAACGATACGCTCGCCa      >  2:156485/1‑76 (MQ=255)                                                                    tCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCAc     >  1:253996/1‑76 (MQ=255)                                                                      tGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCAccc   >  2:557933/1‑76 (MQ=255)                                                                       gCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCa  >  1:200571/1‑76 (MQ=255)                                                                          |                                                                         CAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCA  >  REL606/3251819‑3251964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑