Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,497,639	A→G	F133L (TTC→CTC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,497,639	0	A	G	100.0%	105.4 / NA	28	F133L (TTC→CTC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

GAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCGAG  >  REL606/3497568‑3497711                                                                        |                                                                         gAACATACCGTAGTCATAGCGGATTCCGCAANCGCGCCCCAGCAACNCTAACNTCGCCAGAGAATCGAGGaggcaa                                                                      <  2:156749/76‑1 (MQ=255)     aTACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAgccg                                                                  >  2:570912/1‑76 (MQ=255)     aTACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAgccg                                                                  >  1:232929/1‑76 (MQ=255)                aTAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTccc                                                       >  2:864419/1‑76 (MQ=255)                aTAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTccc                                                       <  2:498715/76‑1 (MQ=255)                 tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCa                                                      <  2:388659/76‑1 (MQ=255)                  aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAg                                                     >  1:195055/1‑76 (MQ=255)                    cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGc                                                   <  1:842250/76‑1 (MQ=255)                      gATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCa                                                 <  1:121185/76‑1 (MQ=255)                        tGCCGTAACCGCGCCCCGGNAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCAcc                                               >  1:1119897/1‑76 (MQ=39)                           cGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGtt                                            >  1:716982/1‑76 (MQ=255)                           cGTAACCGCGCCCCGGAAACCCTAACGACCCCAGAGAATCAAGCAGGCGAGGCGCCAGCCGTCCCAGGCCACCGta                                            >  2:263829/1‑75 (MQ=255)                            gTACCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTa                                           <  1:693391/76‑1 (MQ=255)                             tAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTAc                                          >  1:269673/1‑76 (MQ=255)                              aaCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTAcc                                         <  2:612960/76‑1 (MQ=255)                                ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGCCTTCCCAGGCCACCGTTACCGa                                       <  1:4238/76‑1 (MQ=255)                                  gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg                                     >  2:214495/1‑76 (MQ=255)                                    gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGcc                                   >  2:945501/1‑76 (MQ=255)                                      cccGGCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCg                                 >  2:97931/1‑76 (MQ=255)                                         ggCAACCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGAt                              <  2:1065154/76‑1 (MQ=255)                                         ggCAACCCAAACGACGCCAGAGATTCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGAt                              <  1:1027949/76‑1 (MQ=37)                                            aaCCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCTCCAGACGTCCCAGGCCACCGNTACCGAGGCCCGGATCAt                           <  1:1150404/76‑1 (MQ=38)                                             aCCCTAACGTCGCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCAtt                          <  2:250218/76‑1 (MQ=255)                                                       cGCCAGAGANTCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTtcatca                >  1:33552/1‑76 (MQ=255)                                                        gCCAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaa               >  2:444269/1‑76 (MQ=255)                                                         ccAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaat              <  1:456903/76‑1 (MQ=255)                                                          cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc             <  1:885621/76‑1 (MQ=255)                                                          cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc             <  1:885609/76‑1 (MQ=255)                                                          cAGAGAATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCatcaatc             <  1:806228/76‑1 (MQ=255)                                                             agaATCAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGc          >  1:261737/1‑76 (MQ=255)                                                                  cAAGGAGGCTAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGTATCATTTTCTTCATCAATTAGCTCTTc     <  1:36821/76‑1 (MQ=37)                                                                  cAAGGAGGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTc     <  1:595405/76‑1 (MQ=255)                                                                     ggAGGCAAGCCGCCAGACGTCCCAGGCCACAGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCgag  >  2:952423/1‑76 (MQ=255)                                                                        |                                                                         GAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCCGGATCATTTTCTTCATCAATCAGCTCTTCGAG  >  REL606/3497568‑3497711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑