Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,163,310	A→C	intergenic (+50/‑18)	plsX → / → fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑(acyl carrier protein) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,163,310	0	A	C	100.0%	100.6 / NA	25	intergenic (+50/‑18)	plsX/fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑(acyl carrier protein) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/17);  total (8/17)

GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGAC  >  REL606/1163237‑1163380                                                                          |                                                                       ggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGcaa                                                                      <  2:899860/76‑1 (MQ=255) ggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGcaa                                                                      <  1:864665/76‑1 (MQ=255)    aaaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGt                                                                   >  1:214465/1‑76 (MQ=255)    aaaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGt                                                                   >  2:6921/1‑76 (MQ=255)          ggAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGa                                                             <  2:773827/76‑1 (MQ=255)             aCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCg                                                          <  1:983677/76‑1 (MQ=255)                ctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTAc                                                       >  2:663092/1‑76 (MQ=255)                ctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTAc                                                       <  2:334521/76‑1 (MQ=255)                     gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGta                                                  >  1:521493/1‑76 (MQ=255)                       aGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGtata                                                <  2:343103/76‑1 (MQ=255)                        gCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATAc                                               <  1:263848/76‑1 (MQ=255)                         cAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACg                                              <  2:592803/76‑1 (MQ=255)                          aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCTTACATGTATACGa                                             <  1:83128/76‑1 (MQ=255)                           ggACGCGGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGaa                                            <  2:1183277/76‑1 (MQ=255)                               gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGatt                                        <  2:540182/76‑1 (MQ=255)                               gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGatt                                        >  1:731004/1‑76 (MQ=255)                                 tGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGAGACTGAGCGTACATGTATACGAAGattat                                      >  2:360809/1‑76 (MQ=255)                                       cGAACTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTAc                                <  2:404519/76‑1 (MQ=255)                                         aaCTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTg                              <  2:372292/76‑1 (MQ=255)                                          aCTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTgg                             >  1:779892/1‑76 (MQ=255)                                           cTCGCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGc                            <  2:211342/76‑1 (MQ=255)                                              gCAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGc                         <  2:312032/76‑1 (MQ=255)                                               cAGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCt                        <  1:725078/76‑1 (MQ=255)                                                aggttGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                       <  1:300551/73‑1 (MQ=255)                                                aGTTTGCAAGTGACGGTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                       >  1:505029/1‑76 (MQ=255)                                                               gTATATAACCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGt        <  2:626719/76‑1 (MQ=255)                                                                     aaCCGCAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGAc  <  1:140653/76‑1 (MQ=255)                                                                     aaCCGCAAAGTGACTGAGCGTACATGTATACGAAGATTAATGGTAATGGCAGCTATCTGCCCGAACAAATGCGGAc  <  1:140662/76‑1 (MQ=255)                                                                          |                                                                       GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGAC  >  REL606/1163237‑1163380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑