Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	2,103,915	(CCAG)7→6	coding (182‑185/216 nt)	ECB_01992 →	hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2103892 =	2 (0.040)	35 (1.130)	30/96	0.1	95.5%	coding (159/216 nt)	ECB_01992	hypothetical protein
	?	REL606	= 2103887	2 (0.060)					coding (154/216 nt)	ECB_01992	hypothetical protein

TGTTTTCTTTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103993‑2103892 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctggctggctggctggctggctggctgTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCCATGTTT  <  REL606/2103887‑2103813                                                                                                                                                                                   TGTTTTCTTTTTTANTGTTGCCTTTTATAGTATANTAAGGCCANTTATTTCGTAGTACAAAATCCACATTCACACC                                                                                                       <  2:1269298/76‑1   TTTTCTTTTTTAGTGTTGCCTTGTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTG                                                                                                     <  2:54146/76‑1      TCTTTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCT                                                                                                  >  2:1035008/1‑76         TTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGC                                                                                               >  1:1217964/1‑76          TTTTAGTGATGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACGTGGCTGGCT                                                                                              >  1:45687/1‑76           TTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTG                                                                                             >  2:1409558/1‑76            TTAGTGTTGCCTTTTATAGTATAATGAGGGCGATTATTTCNNAGTACAAAATNNATATTCACACCTGGCTGGCTGG                                                                                            >  1:1379896/1‑76                TGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGG                                                                                        >  2:173884/1‑76                TGTTGCCTNTTATAGTATANTAAGGGCAATTATTTCGTAGTANAAAATCCACATTCACACCTGGCTGGCTGGCTGG                                                                                        <  2:1286970/76‑1                 GTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGC                                                                                       >  1:707390/1‑76                  TTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCT                                                                                      >  1:386912/1‑76                   TGCCTTTTATAGTATAATAAGTGGAATTATTTCGTAGTACAAAATCCACATTCACGCCTGGCTGGCTGGCTGGCTG                                                                                     <  2:113738/76‑1                   TGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTG                                                                                     <  2:809714/76‑1                   TGCCTTTTATAGTATAATAAGGGCAATAATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTG                                                                                     >  1:1517085/1‑76                         TTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGC                                                                               >  1:896496/1‑76                         TTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGC                                                                               >  1:896494/1‑76                          TATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCT                                                                              <  2:1316601/76‑1                          TATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCT                                                                              >  1:1220681/1‑76                            TAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGT                                                                            <  1:1039635/76‑1                             AGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTG                                                                           <  2:1366045/76‑1                               TATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAA                                                                         >  1:757642/1‑76                                ATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTG                                                                                 <  2:473489/67‑1                                ATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTG                                                                                 >  1:473489/1‑67                                    TAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTA                                                                    <  1:1323459/76‑1                                     AAGTGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAA                                                                   <  2:405644/76‑1                                     AAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAA                                                                   >  2:1247504/1‑76                                     AAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGGAAATCTAA                                                                   <  2:1307811/76‑1                                     AAGGGCAATTATTNCGTAGTACAAAATCNACNATCACACCTGGCTGGCGGGCTGGCTGGCTGGCTGTGAAATCTAA                                                                   <  2:1383739/76‑1                                       GGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATG                                                                 <  1:497575/76‑1                                         GCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCA                                                               >  2:955877/1‑76                                          CAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAA                                                              >  2:719767/1‑76                                           AATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAA                                                             >  2:81699/1‑76                                            ATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAAT                                                            >  2:1235939/1‑76                                             TTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATT                                                           >  2:329756/1‑76                                              TATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTT                                                          >  2:66969/1‑76                                                 TTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC                                                       <  2:548045/76‑1                                                 TTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC                                                       >  1:1035759/1‑76                                                    GTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCC                                                    >  2:90861/1‑76                                                    GTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATATAATGCAAATTTCACCCC                                                    >  1:694161/1‑76                                                     TAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCT                                                   >  1:472259/1‑76                                                     TAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCT                                                   >  2:723003/1‑76                                                      AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTT                                                  <  2:1214559/76‑1                                                      AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTATGAAATCTAATGCAAATTTCACCCCTT                                                  >  1:461741/1‑76                                                         ACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGCGAAATCTAATGCAAATTTCACCCCTTTAT                                               >  2:1117048/1‑76                                                           AAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATAT                                             >  2:1166474/1‑76                                                            AAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATT                                            <  2:137662/76‑1                                                              ATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAACTCTAATGCAAATTTCACCCCTTTATATTCG                                          <  1:1392083/76‑1                                                              ATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCG                                          <  2:396358/76‑1                                                               TCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGA                                         <  1:233674/76‑1                                                                 CACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAG                                       <  1:119890/76‑1                                                                   CATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTC                                     <  2:3882/76‑1                                                                    ATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCA                                    <  1:236300/76‑1                                                                      TCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAAT                                  >  2:844627/1‑76                                                                       CACACCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATA                                 <  2:63325/76‑1                                                                           CCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGA                             <  2:757642/76‑1                                                                            CTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAA                            <  2:837327/76‑1                                                                            CTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAA                            >  1:416276/1‑76                                                                            CTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAANCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAANGAA                            >  2:1424650/1‑76                                                                             TGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAG                           >  1:511534/1‑76                                                                             TGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAG                           >  2:929040/1‑76                                                                              GGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTAGAAGTCAATAACGAAGC                          >  1:174489/1‑76                                                                                    CTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTT                    <  2:633286/76‑1                                                                                     TGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTC                   >  2:914859/1‑76                                                                                      GGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCA                  <  1:292405/76‑1                                                                                         TGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTG               >  2:475168/1‑76                                                                                         TGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTG               >  2:343699/1‑76                                                                                          GGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGT              >  1:246988/1‑76                                                                                          GGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGT              <  1:1269038/76‑1                                                                                             TGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA           <  2:977415/76‑1                                                                                             TGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA           <  2:303447/76‑1                                                                                              GGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAG          <  1:1116721/76‑1                                                                                                CTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCC        >  2:929641/1‑76                                                                                                 TGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCCA       >  2:969760/1‑76                                                                                                     TGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCCATGTT   >  2:374719/1‑76                                                                                                      GTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCCATGTTT  <  1:675708/76‑1                                                                                                                                                                                   TGTTTTCTTTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103993‑2103892 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctggctggctggctggctggctggctgTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATAGCCATGTTT  <  REL606/2103887‑2103813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.