| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 97.7% | 142.9 / NA | 43 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/23); minor base C (0/1); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193710‑2193851 | aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:671484/76‑1 (MQ=255) tGCATCGGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCGAATAACAGCAAAACGCCAATCCccacc < 1:1518247/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:1117515/76‑1 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca < 2:457456/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa > 1:611440/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:1473625/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa > 2:1341539/1‑76 (MQ=255) gCGTCCAGGCGCGGCGGGTCTGTCGGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:53678/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAATCAGACCCAATAACAGCAAAACGCCNATCCCCACCAGATAAAGc < 1:1462705/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:1212046/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGCTGTCNGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc > 1:1536958/1‑76 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGcn < 1:150477/76‑2 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 1:1041660/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:1072953/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:566387/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa > 2:1532770/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAACCA‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 2:949241/76‑1 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 2:848020/1‑76 (MQ=255) ggCNGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACNCCAATCCCCACCAGATAAAGCATGACGCCTGt < 2:1430804/76‑1 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:838525/76‑1 (MQ=255) ggTCAGGCTGTCGGAAAACAGCCCCAATAACAGCAAAACGCCAATCCCCAGCAGATAAAGCATGTCGCCGGTANTa > 2:1441108/1‑76 (MQ=255) gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:594539/76‑1 (MQ=255) ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:903300/76‑1 (MQ=255) tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 2:644548/1‑76 (MQ=255) gTCGGAAATCAGCNCCAATAACAGCAAAACGCCAATCCCCACCAGGTAAAGCATGACGCCTGTATTAAACAGGCtt < 2:1391510/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:664377/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:733459/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:1250829/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCCCCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 1:728709/76‑1 (MQ=255) gAAATCAGCTCCAATAACAGCAANACGCCNATCCCGACCAGATANAGAATGACGCCTGTATTAAACAGGCTTTTTa > 2:199628/1‑76 (MQ=255) cAGCCCCAATAACAGCAAAAGGACAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 1:12284/1‑76 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:988557/76‑1 (MQ=255) aGCCTCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACCCCTGTATTAAACAGGCCTTTTGCCGNTg > 2:1441303/1‑76 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:723473/1‑76 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg > 2:906191/1‑76 (MQ=255) gattAACAGCAAANCGCCAATCCCGACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 1:199628/73‑1 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 2:281707/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:951298/1‑76 (MQ=255) cAGCAAAACGCCAATCCCCACCAGATAAAACATGACCCCTGTATTAAACAGGCTTTTNACCGATGGCGCATCgcgc < 1:1457511/76‑1 (MQ=255) aaaaCGCCAATCCCCACTAGATAAAGCATGACGCCTGTATTAAACAGGCTATTTACCGATGGCGAATCGCGCCNCa > 2:1444485/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 2:140688/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:1334087/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:431455/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:450559/1‑76 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca < 1:776348/76‑1 (MQ=255) | ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193710‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |