| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,873,434 | T→G | T201P (ACC→CCC) | glmS ← | D‑fructose‑6‑phosphate amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,873,434 | 0 | T | G | 97.2% | 139.4 / ‑6.7 | 36 | T201P (ACC→CCC) | glmS | D‑fructose‑6‑phosphate amidotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (18/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAG > REL606/3873361‑3873500 | ttATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCa > 1:839322/1‑76 (MQ=255) aTCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCAcc > 2:1095307/1‑76 (MQ=255) aTCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCAcc > 2:936272/1‑76 (MQ=255) tCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCg < 1:453967/76‑1 (MQ=255) tCGAAGATGTTTACCGAACGACNNGTGATTTCCGNNTTATCGCCCTCTTCGAGGAAGATAAAGCGACGGGGCACCg < 1:1380181/76‑1 (MQ=255) cGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCgg > 1:1329668/1‑76 (MQ=255) gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGc < 2:1014976/76‑1 (MQ=255) gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGc < 2:1014970/76‑1 (MQ=255) tGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAg < 2:93993/76‑1 (MQ=255) aCCGAACGGCGAGTGCTTTCCTTAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCa < 2:46138/76‑1 (MQ=255) aCCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCa < 2:653457/76‑1 (MQ=255) ccGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCGAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAg < 2:1360717/76‑1 (MQ=255) gAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGcc > 2:146903/1‑75 (MQ=255) aaCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTg < 2:37618/76‑1 (MQ=255) aCGGCGAGTGAGTTCCGCAATATCGCCCGCTTCAAGGAAGATAAAGCGTCGGGGCACCGGCAACAGCGCCAGCTgg < 2:131175/76‑1 (MQ=255) gAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGa > 1:1115632/1‑76 (MQ=255) gAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGa > 1:1023666/1‑76 (MQ=255) aGTGATTTCCGCAATATCGCCCTCTTCCAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGCTCAGaa > 1:1510478/1‑76 (MQ=255) gTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAg < 1:892008/76‑1 (MQ=255) gATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCg < 2:864347/76‑1 (MQ=255) tttCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGAt > 1:1117469/1‑76 (MQ=255) ttCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATa > 2:568170/1‑76 (MQ=255) cAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt > 2:475204/1‑76 (MQ=255) atatCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt < 2:827032/76‑1 (MQ=255) cGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGcc > 2:416218/1‑76 (MQ=255) gCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGccc > 1:1129492/1‑76 (MQ=255) cccTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCa < 2:1364795/76‑1 (MQ=255) ccTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCAt > 1:1115385/1‑76 (MQ=255) ttCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc < 2:1534528/76‑1 (MQ=255) ttCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc > 1:650941/1‑76 (MQ=255) aaGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCaa < 2:650684/76‑1 (MQ=255) gATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc < 2:1107339/76‑1 (MQ=255) tAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCATGCCAATCAc > 1:54448/1‑76 (MQ=255) tAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAc < 1:1219709/76‑1 (MQ=255) aaaGCGACGGGGCACCGGCAACAGCGCCAGCTGNTCAGAAGCGATAAAGTTTTCGCNCATCCCCAAGNCAATCAcc > 2:1276458/1‑76 (MQ=255) aaGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCa > 2:589177/1‑76 (MQ=255) aGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAg > 2:385360/1‑76 (MQ=255) | TTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAG > REL606/3873361‑3873500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |