| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,317 | Δ1 bp | coding (989/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,315 | 0 | G | . | 96.4% | 125.3 / ‑2.1 | 28 | coding (991/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (12/15); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGT > REL606/474242‑474384 | cccAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTgga < 1:647683/76‑2 (MQ=255) ccAGTCCCGCCAGGATAGACGCGGACTCACCAATCTGCGCCAGGCTTGCGGCGATGGTTAATGCCGTAGGTTggag > 1:71859/1‑74 (MQ=255) ccAGTCCCGCCAGGATAAACGCGAACTCACGAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTggag > 1:1303712/1‑74 (MQ=255) ccAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTggag > 1:1150785/1‑74 (MQ=255) ccGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCa < 1:385728/76‑1 (MQ=255) gATAAACGCGACCCCACCAACCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTc < 1:522704/76‑1 (MQ=255) gATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTc < 1:337533/76‑1 (MQ=255) tAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑TGAGTGACCAAACAGTCGc < 1:977348/76‑1 (MQ=255) tAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGc < 1:1081235/76‑1 (MQ=255) aaaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCa > 2:468262/1‑76 (MQ=255) aaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCAc < 2:1034499/76‑1 (MQ=255) cgAACTCACCAATNTGCGCCAGGCTGGCGGCGATGGTTAATGCCGAACGTT‑GGAGTGACCAAACAGTCGCACCAgg < 2:1298864/76‑1 (MQ=255) gAACTCACCGATCTGCGCCAGGCTGGCGGCGATCGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGa > 2:1228655/1‑76 (MQ=255) aCTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGaaa < 1:479366/76‑1 (MQ=255) cTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGaaaa < 1:774788/76‑1 (MQ=255) cACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAAt > 2:1156138/1‑76 (MQ=255) ccAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGc > 2:69950/1‑76 (MQ=255) aaTCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCgg > 1:737854/1‑76 (MQ=255) cTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTa > 2:687284/1‑76 (MQ=255) cTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTa < 2:396979/76‑1 (MQ=255) gcgcCAGGCTGGCGGCGATGGTTAATGCCGCACGTT‑GGAGAGACCCAACAGTCGCACCAGGANAAATGCGGCTAAc > 2:1308281/1‑76 (MQ=255) cgcCAGGCTGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACg < 2:975468/76‑1 (MQ=255) tGGCGGCGATGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCa < 1:1263207/76‑1 (MQ=255) ggcggcGATGGTTAATGTAGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAANGCGGCTAACGACTTACCaa < 1:1328930/76‑1 (MQ=255) gcgATGGTTAATGCCGTACGTT‑GGAGTAACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAg < 1:572578/76‑1 (MQ=255) aTGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaa > 2:443404/1‑76 (MQ=255) aTGGTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaat > 2:693441/1‑76 (MQ=255) ggTTAATGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaataa > 1:235763/1‑76 (MQ=255) aTGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGcc > 1:1200838/1‑76 (MQ=255) aTGCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGcc < 1:951054/76‑1 (MQ=255) gCCGTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAg < 2:85105/76‑1 (MQ=255) cGTACGTT‑GGAGTGACCAAACAGTCGCAACAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCCCTAGCg > 1:1408842/1‑76 (MQ=255) gTACGTT‑GGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGAGt > 2:409477/1‑76 (MQ=255) | CCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGT > REL606/474242‑474384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |