| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,944 | C→T | A243V (GCG→GTG) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,944 | 0 | C | T | 98.2% | 200.4 / ‑3.6 | 55 | A243V (GCG→GTG) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (25/29); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
ATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAT > REL606/3928871‑3929016 | aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTgg > 2:988066/1‑76 (MQ=255)aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTgg < 1:640681/76‑1 (MQ=255) tGCAGCGTCTGGTAAAAGGAGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGCTGTGGa > 1:760788/1‑76 (MQ=255) cAGCGTCTGGTAANAGGTGAAGTTGTTGCTTCTACGTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGaaa < 2:1305100/76‑1 (MQ=255) cGTCTGGTAAAAGGTGAAGTTGTTGTTTCTAGCTTTGACTNNCCCGCATCTCNNCGCGTTGAGGTTGTAGAAATgg > 1:1283499/1‑76 (MQ=255) gTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGt > 1:315067/1‑76 (MQ=255) tCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTg < 2:1309644/76‑1 (MQ=255) tCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTg < 2:1061557/76‑1 (MQ=255) ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATc < 2:676184/76‑1 (MQ=255) ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATc < 2:585289/76‑1 (MQ=255) ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTAGCCACGTTCAGGTTGTGGAAACGGTGATc > 1:72674/1‑76 (MQ=255) gTAAAATGTGAAATTGTTGCTACTGCCTATGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCg < 2:242632/76‑1 (MQ=255) tAAAAGGTGAAGTAGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCga > 1:101411/1‑76 (MQ=255) aaaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCgag > 1:1241360/1‑76 (MQ=255) aaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCgaga < 2:288625/76‑1 (MQ=255) aaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGaa > 2:1230602/1‑76 (MQ=255) ggTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGCTCAGGTTGTGGAAATGGTGATCGAGAAgg > 1:311099/1‑76 (MQ=255) gTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGc < 1:539290/76‑1 (MQ=255) gTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGc > 1:797522/1‑76 (MQ=255) gAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCa < 2:1378822/76‑1 (MQ=255) aaGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCaa < 1:623248/76‑1 (MQ=255) aGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCaaa > 1:642109/1‑76 (MQ=255) ttgttgCTTCTACCTTTGACGACCCCGCATCTCGCCACGTTCAGGTTGTGGAAAAGGTGATAGAGAAGGCCAAACg > 2:213117/1‑76 (MQ=255) ttgttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACg < 1:216753/76‑1 (MQ=255) tgttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGc > 1:410700/1‑76 (MQ=255) gttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGcc > 1:581230/1‑76 (MQ=255) gttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGcc > 1:687544/1‑76 (MQ=255) ttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCt > 1:862432/1‑76 (MQ=255) tgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTg < 2:1135845/76‑1 (MQ=255) ttCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt > 1:462412/1‑76 (MQ=255) ttCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt < 2:942920/76‑1 (MQ=255) tCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGt < 2:722939/74‑1 (MQ=255) tCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGt > 1:722939/1‑74 (MQ=255) cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGcac < 2:672780/76‑1 (MQ=255) ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 2:1074890/76‑1 (MQ=255) ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 1:213117/76‑1 (MQ=255) ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 2:872029/76‑1 (MQ=255) tGACGAACCCGCATCTCGCNACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaag > 1:1334997/1‑76 (MQ=255) gACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaaga < 1:978387/76‑1 (MQ=255) gACGAACCCGCATCTCGCCACGTACAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaaga < 1:788766/76‑1 (MQ=255) aCGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaagaa < 1:566495/76‑1 (MQ=255) aaCCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaagaaaga < 1:1197196/76‑1 (MQ=255) aCCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGAc > 2:647489/1‑76 (MQ=255) cGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGtt < 2:427633/76‑1 (MQ=255) cATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTAt < 1:240766/76‑1 (MQ=255) aTCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGATTGAGCACACGAAAGACGTTATc > 1:1229869/1‑76 (MQ=255) tctcGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCa < 1:113646/76‑1 (MQ=255) tctcGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCa < 2:601509/76‑1 (MQ=255) gCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCt < 2:1378542/76‑1 (MQ=255) cccACGTTCAGGTTGTGGAAATGGTGATGGAGAATGCCAAACGCCTGNTTAAGCACAAGAAAGACGTTATCATTCt > 1:124079/2‑76 (MQ=255) cACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGc > 2:619913/1‑76 (MQ=255) gTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCg < 2:6599/76‑1 (MQ=255) tCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGAc > 1:1051845/1‑76 (MQ=255) cAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACt > 1:1081383/1‑76 (MQ=255) gTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCa < 2:760788/76‑1 (MQ=255) gTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCa > 2:58992/1‑76 (MQ=255) ttGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAt > 1:583505/1‑76 (MQ=255) | ATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAT > REL606/3928871‑3929016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |