Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,167,668	T→C	L903P (CTG→CCG)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,167,668	0	T	C	100.0%	203.0 / NA	49	L903P (CTG→CCG)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

GGTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGG  >  REL606/4167597‑4167740                                                                        |                                                                         ggTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGgcg                                                                      >  2:702189/1‑76 (MQ=255)  gTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCTCTGCTACGGTCGTGACCCGgcgc                                                                     >  1:1224511/1‑76 (MQ=255)   ttAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGcgcg                                                                    >  1:289791/1‑76 (MQ=255)     aaaGTACGTTCTGTTGTATNTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGTTCGTGACCCGGCGCGTg                                                                  >  1:1326627/1‑76 (MQ=255)     aaaGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTg                                                                  >  2:1244561/1‑76 (MQ=255)     aaaGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTg                                                                  <  2:1144080/76‑1 (MQ=255)      aaGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTgg                                                                 >  2:29594/1‑76 (MQ=255)      aaGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTgg                                                                 >  2:242573/1‑76 (MQ=255)         tACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCca                                                              <  2:595598/76‑1 (MQ=255)          aCGTTCTGTTGTNTCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCcac                                                             >  1:13164/1‑76 (MQ=255)           cGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCcaca                                                            >  1:1027662/1‑76 (MQ=255)           cGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCcaca                                                            >  1:263895/1‑76 (MQ=255)            gTTCTGTTATATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCTTGGCCAcat                                                           >  1:49094/1‑76 (MQ=255)              tCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCAcatca                                                         >  1:484748/1‑76 (MQ=255)                tgttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACatcatc                                                       <  1:804988/76‑1 (MQ=255)                 gttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACCtcatca                                                      <  1:81856/76‑1 (MQ=255)                 gttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACAtcatca                                                      <  1:735280/76‑1 (MQ=255)                 gttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACAtcatca                                                      >  1:416251/1‑76 (MQ=255)                  ttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATcaa                                                     <  1:425838/76‑1 (MQ=255)                   tgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATcaac                                                    <  1:471777/76‑1 (MQ=255)                     tATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATcaacaa                                                  <  1:217630/76‑1 (MQ=255)                       tCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATTATCAACAAgg                                                <  1:1312784/76‑1 (MQ=255)                         ttGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGt                                              >  2:146737/1‑76 (MQ=255)                          tgtgACACCTACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTg                                             >  1:923307/1‑76 (MQ=255)                           gtgACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGa                                            <  1:485770/76‑1 (MQ=255)                           gtgACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGa                                            <  2:610553/76‑1 (MQ=255)                           gtgACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGa                                            <  2:1316723/76‑1 (MQ=255)                                 ccGNCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCNCGTGGCCACATCATCAACAAGGGTGAAGCAAt                                      <  2:1326627/76‑1 (MQ=255)                                 ccGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAAt                                      >  1:687630/1‑76 (MQ=255)                                  cGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATc                                     <  1:97995/76‑1 (MQ=255)                                    aCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCgg                                   >  2:736887/1‑76 (MQ=255)                                    aCTTTGGTGTATATGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCgg                                   <  2:804187/76‑1 (MQ=255)                                           tgtATGTGCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATc                            >  2:584694/1‑76 (MQ=255)                                                gtgCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGc                       <  1:1378595/76‑1 (MQ=255)                                                 tgCGCACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGAca                      >  2:607452/1‑76 (MQ=255)                                                   cgcACTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGcaca                    <  1:296959/76‑1 (MQ=255)                                                      ccTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTc                 <  2:687630/75‑1 (MQ=255)                                                       cTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTcc                >  2:516965/1‑76 (MQ=255)                                                       cTGCTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTcc                <  2:123936/76‑1 (MQ=255)                                                         gCTACGGTCGTGACCCGGGGCATGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCAt              <  1:1342424/76‑1 (MQ=255)                                                          cTACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATc             >  2:681584/1‑76 (MQ=255)                                                           tACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCg            >  1:588440/1‑76 (MQ=255)                                                           tACGGTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCg            >  1:584608/1‑76 (MQ=255)                                                               gTCGTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGa        >  1:404002/1‑76 (MQ=255)                                                                  gTGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAAcc     <  2:971739/76‑1 (MQ=255)                                                                   tGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATTGGTGAACCg    >  1:803779/1‑76 (MQ=255)                                                                   tGACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCg    <  2:113435/76‑1 (MQ=255)                                                                    gACCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCgg   >  1:1104988/1‑76 (MQ=255)                                                                     aCCCGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCggg  <  2:1002166/76‑1 (MQ=255)                                                                        |                                                                         GGTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGG  >  REL606/4167597‑4167740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑