Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,464	T→A	D259V (GAT→GTT)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,464	0	T	A	100.0%	130.5 / NA	35	D259V (GAT→GTT)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/15);  total (20/15)

TCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGT  >  REL606/1305392‑1305533                                                                         |                                                                      tCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACaa                                                                    >  2:69453/1‑76 (MQ=255)  cAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAAt                                                                   >  1:297102/1‑76 (MQ=255)  cAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAAt                                                                   >  1:708827/1‑76 (MQ=255)    aTCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATcc                                                                 >  2:789777/1‑76 (MQ=255)      cccccAGTCGCAGGAATAAATAACCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCAt                                                               <  2:124525/74‑1 (MQ=255)        cccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTg                                                             >  1:1162890/1‑76 (MQ=255)        cccAATCGAAGGAATAAATAGCCCCCATCGCGTGGGCGGTGGGGCCTTCCATACGCGCCATCAGAACAATCCATTg                                                             <  2:1385622/76‑1 (MQ=255)              cGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTAc                                                       >  2:725366/1‑76 (MQ=255)              cGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTAc                                                       >  2:668507/1‑76 (MQ=255)                cAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGc                                                     >  2:13169/1‑76 (MQ=255)                  ggAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGccc                                                   >  1:304913/1‑76 (MQ=255)                   gAATAAATAACCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCg                                                  <  2:370098/76‑1 (MQ=255)                      taaataaTCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCAt                                               >  2:1420521/1‑76 (MQ=255)                           aacccccAGCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGt                                          <  2:32940/73‑1 (MQ=255)                             tCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGttt                                        >  2:683135/1‑76 (MQ=255)                             tCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGttt                                        >  2:1094856/1‑76 (MQ=255)                             cccccACCGCGGGGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGttt                                        <  2:705041/75‑1 (MQ=255)                                  aTCGCGGTGGCGATGGGGCNTTCCATACGCGCCATCAGAACAATCCTTTGCCCTACGCTCGCATCTTGTTTGAAGt                                   >  1:1328360/1‑76 (MQ=255)                                    cgcgGTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAg                                 >  2:484701/1‑76 (MQ=255)                                        gTGGCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAg                             >  1:48115/1‑76 (MQ=255)                                          ggCGATGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAggg                           >  2:250898/1‑76 (MQ=255)                                             gagggggCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCg                        <  1:1090965/73‑1 (MQ=255)                                               tGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGAc                      >  1:1372360/1‑76 (MQ=255)                                               tGGGGCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGAc                      >  2:268205/1‑76 (MQ=255)                                                ggggCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGAcc                     >  1:706309/1‑76 (MQ=255)                                                gggcCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGAcc                     <  1:407704/76‑1 (MQ=255)                                                 gggCCTTCCATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCa                    <  2:1139341/76‑1 (MQ=255)                                                   gCCTTCCATACGCGCCATCAGAACAATCCATAGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCata                  <  2:247793/76‑1 (MQ=255)                                                     cTTCCATACGCGCCATCAGAACAATCCATAGCCCTACGCCCGCATCTTGTGTGAAGTATCGAGGGTCGACCATAtt                <  1:78173/76‑1 (MQ=255)                                                        ccATACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAt             <  2:841355/76‑1 (MQ=255)                                                          aTACGCGCCCTCAGAACAATCCANTGCCCNACGCCAGCATCTTGNTTGAAGCAGCGAGGGTTGACCATATTCAtgc           >  2:191646/1‑76 (MQ=255)                                                           tACGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgct          <  1:554944/76‑1 (MQ=255)                                                            aCGCGCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgctg         <  1:421249/76‑1 (MQ=255)                                                             cgcgCCATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgctgc        <  1:818564/76‑1 (MQ=255)                                                                 ccATCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGgt    <  2:417542/76‑1 (MQ=255)                                                                   aTCAGAACAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGgtgt  >  1:776627/1‑76 (MQ=255)                                                                   aTCAAAACAATCCATTCCCCTCCGCCGCCATCTTGTTTGAAGTAGCGAGGGTCGCCCATATTCATCCTGCCGgtgt  <  2:150441/76‑1 (MQ=255)                                                                         |                                                                      TCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGT  >  REL606/1305392‑1305533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑