Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	101.9 / NA	29	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

TCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329447‑1329588                                                                      |                                                                         tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  1:419009/1‑76 (MQ=255)   cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgc                                                                  >  1:1011337/1‑76 (MQ=255)    ccagCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  2:106060/73‑1 (MQ=255)    ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  1:262309/76‑1 (MQ=255)     cGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGa                                                                >  2:553188/1‑76 (MQ=255)        cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:1126354/76‑1 (MQ=255)        cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:648027/76‑1 (MQ=255)        cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:1126337/76‑1 (MQ=255)        cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:648013/76‑1 (MQ=255)          aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:60953/1‑76 (MQ=255)          aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:364795/1‑76 (MQ=255)                 aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  2:341510/1‑76 (MQ=255)                         aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            >  1:443874/1‑76 (MQ=255)                          ncaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCNACCAGTGGCTCAGc                                           >  2:201833/2‑76 (MQ=255)                            aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  1:528962/1‑76 (MQ=255)                                 tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaa                                    >  1:303619/1‑76 (MQ=255)                                     ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCANCTGCCAAAAaga                                <  1:18123/76‑1 (MQ=255)                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             <  2:336509/76‑1 (MQ=255)                                              tACGTGTTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCGGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                       <  1:1296822/76‑1 (MQ=255)                                              tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                       >  1:743851/1‑76 (MQ=255)                                                cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  1:941481/76‑1 (MQ=255)                                                 gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  1:731476/76‑1 (MQ=255)                                                 gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:741378/76‑1 (MQ=255)                                                           ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          >  1:789939/1‑76 (MQ=255)                                                            cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:516967/76‑1 (MQ=255)                                                            cAGCGTCGGTTACATCCGAGAATTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:1350773/76‑1 (MQ=255)                                                                 tCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGc    <  2:1058511/76‑1 (MQ=255)                                                                 tCGGTTACATCCGCGATTTGCCAACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGc    <  2:1079233/76‑1 (MQ=255)                                                                  cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  1:918349/76‑1 (MQ=255)                                                                   ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  >  1:457038/1‑76 (MQ=255)                                                                      |                                                                         TCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329447‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑