| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,886,183 | A→G | intergenic (‑13/+656) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,886,183 | 0 | A | G | 92.2% | 178.9 / 1.6 | 51 | intergenic (‑13/+656) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (26/21); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGAT > REL606/1886111‑1886255 | gaAGCGGTATTCAACGTCAACGTGTTTAGTCAGGCCAACTTTACTGANAATGCCACACATAAACAGAACTGTGCCt > 1:123291/1‑76 (MQ=255) aaGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTc > 2:1417537/1‑76 (MQ=255) aaGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTc > 2:1417546/1‑76 (MQ=255) aGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCg < 2:263437/76‑1 (MQ=255) ggTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGttt < 2:10015/76‑1 (MQ=255) gTATTCAANGGCAACGTGTNTACTCAGGACTTCTTTACTGAANATGCCACACATAAACAGAACTGTGCCTCGTTTa < 2:1194531/76‑1 (MQ=255) caacgtcaatgtGTTTACTCAGGNCTTCTNTACTGAAAATGCCANACTTAAACAGAACTGTACCTCGTTTAACCCg > 2:187244/1‑76 (MQ=255) aacgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGa < 2:70384/76‑1 (MQ=255) aacgtcaacgtGTTTACTCAGGACTTCTTTACNGACAATGCNACACATAAACAGAACTGTGNCTCGTTTAACCCGa > 2:1178759/1‑76 (MQ=255) cgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGaaa > 2:5767/1‑76 (MQ=255) gtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAAt < 2:90447/76‑1 (MQ=255) tcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATc < 2:510075/76‑1 (MQ=255) aacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTg > 2:260550/1‑76 (MQ=255) tgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTAt < 2:590654/76‑1 (MQ=255) tgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTAt < 1:834707/76‑1 (MQ=255) gtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATc > 1:1119214/1‑76 (MQ=255) tttACTCAGGACTTCTTTGCTGAAAATGCCACACATAAACAGAANTGNGCCTCGTTTAACCCNAAATCTGTTATca > 2:1277834/1‑76 (MQ=255) tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAANTGNGCCTCGTTTAACCCNAAATCTGTTATca > 2:1277829/1‑76 (MQ=255) tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATca > 2:138837/1‑76 (MQ=255) tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATca < 2:123291/76‑1 (MQ=255) ttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATcac > 2:471672/1‑76 (MQ=255) aCTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACAt < 1:159704/76‑1 (MQ=255) cTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTTTGCCTCGTTTAACCCGAAATCTGTTATCACATa < 1:1085268/76‑1 (MQ=255) cTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATa > 1:1301764/1‑76 (MQ=255) cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACa < 1:569026/76‑1 (MQ=255) gACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACg > 1:327855/1‑76 (MQ=255) ttcttTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTa < 2:839830/76‑1 (MQ=255) tcttTACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTaa > 2:706174/1‑76 (MQ=255) tcttTACTG‑AAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAAt < 1:1107243/76‑1 (MQ=255) tttACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAAtt > 1:723465/1‑76 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTAt > 2:78934/1‑76 (MQ=255) aCTGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATg > 1:770327/1‑76 (MQ=255) tGAAAATGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGc < 1:1021397/76‑1 (MQ=255) gAAAATGCCACACATAAACAGAACTGAACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 1:946141/76‑1 (MQ=255) aaaaTGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCaa > 1:304980/1‑76 (MQ=255) aaaTGCCACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAg > 2:785668/1‑76 (MQ=255) aaTGCCACACGTACACNGCACTGTGCCTCGCTNAACCCGACATCTGTTATCACATACAACGTTAANTATGGCNANc > 1:106120/1‑76 (MQ=255) tccACACATAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAt > 2:837552/2‑76 (MQ=255) cacacaTAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAttt < 1:1265775/76‑1 (MQ=255) acacaTAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAtttt > 1:979041/1‑76 (MQ=255) caTAAACAGAACTNTGCCTCGTTTAACCCGAAATCTGTTATCACAGACAACGTTAATTATGGCAAGCCATTTTTaa < 2:1295936/76‑1 (MQ=255) caTAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa < 2:342589/76‑1 (MQ=255) tAAACAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAAc > 2:887804/1‑76 (MQ=255) aaaCAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACa > 2:779754/1‑76 (MQ=255) aaCAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACAt > 2:505227/1‑76 (MQ=255) cAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGt > 2:549125/1‑76 (MQ=255) cAGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGt < 1:870637/76‑1 (MQ=255) aGAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGtc < 2:407589/76‑1 (MQ=255) gAACTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGtct < 2:723465/76‑1 (MQ=255) aCTGTGCCTAGTTTAACCCGAAANCTGTTNTCACACACAACGTTNATAATGACAAGCCATTATTAAACATGTCTCg > 2:184097/1‑76 (MQ=255) cTGTGCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGa < 2:420297/76‑1 (MQ=255) tgtgCCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGAt > 1:1033920/1‑76 (MQ=255) | GAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGAT > REL606/1886111‑1886255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |