| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,165,181 | C→A | G221C (GGC→TGC) | yehU ← | predicted sensory kinase in two‑component system with YehT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,165,181 | 0 | C | A | 97.4% | 127.7 / NA | 38 | G221C (GGC→TGC) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (21/16); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCCAGCTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCCTTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTT > REL606/2165109‑2165253 | tatCCAGCTCCTGATACAGCACCTGAGCCACTTTCAGGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTc < 1:368837/76‑1 (MQ=255) atCCAGCTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTcc < 1:209454/76‑1 (MQ=255) tCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAgg > 2:958688/1‑76 (MQ=255) tGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTg > 2:7363/1‑76 (MQ=255) aTACAGCACCTGAGCCACCTTCATGCTGTTCACTTCGTTAAACCCCTGTGGCAAAATGCCTTCCGTCGAGGCTGcc < 1:1347540/76‑1 (MQ=255) tACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGccc > 2:16323/1‑75 (MQ=255) cTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAgc > 1:985303/1‑76 (MQ=255) tGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAgcg > 2:276342/1‑76 (MQ=255) tGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAgcg > 1:553652/1‑76 (MQ=255) cACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTg < 2:514532/76‑1 (MQ=255) cACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTg < 1:650234/76‑1 (MQ=255) ttCATGCTGTTCACTTCGTTAGCCCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAg < 1:754705/76‑1 (MQ=255) ttCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAGATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAg < 1:1081659/76‑1 (MQ=255) ttCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAg < 2:230916/76‑1 (MQ=255) ttCATGCTGTTCACNTCGTTAACCCCCTCTCGCANAATGCGTTNCGTCGAGGCTGCCACTTTCCGCGCAGTGGCAg < 2:1172749/76‑1 (MQ=255) tCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGa > 1:964224/1‑76 (MQ=255) cATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGaa < 2:1419451/76‑1 (MQ=255) tGCTATTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGaaaa > 2:1404685/1‑76 (MQ=255) gCTGTTCACTTCGTTAACCCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGaaaaa < 1:591222/76‑1 (MQ=255) gCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGaaaaa < 1:636598/76‑1 (MQ=255) tGTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGACNCTNCCACTTTCAGCGCANTGGCAGAAAAAGc > 2:1286259/1‑76 (MQ=255) gTTCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGcc < 2:427765/76‑1 (MQ=255) ttCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCg < 1:1213454/76‑1 (MQ=255) tCACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGa > 2:56995/1‑76 (MQ=255) cACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGaa > 1:1088323/1‑76 (MQ=255) cACTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGaa < 1:1212354/76‑1 (MQ=255) cTTCGTTAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAgg > 1:362143/1‑75 (MQ=255) ttAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAtt > 1:879622/1‑76 (MQ=255) tAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAttt > 2:1092374/1‑76 (MQ=255) tAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAttt > 2:483746/1‑76 (MQ=255) tAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAttt > 2:703791/1‑76 (MQ=255) tAAACCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAttt > 2:967361/1‑76 (MQ=255) aacccccTGTCGCAAAATGCATCCGGCCGGGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAtttt < 1:1139203/73‑1 (MQ=255) aaCCCCTGTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTAttttt > 1:143920/1‑76 (MQ=255) gTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACAt < 1:148902/76‑1 (MQ=255) gTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACAt < 1:1145545/76‑1 (MQ=255) gTCGCAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCAGAAGTGTATTTTTCAAACAt > 2:101699/1‑76 (MQ=255) cAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATcgcg < 1:784385/76‑1 (MQ=255) cAAAATGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATcgcg > 2:274145/1‑76 (MQ=255) aaTGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGt > 2:880274/1‑76 (MQ=255) tGCATTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGAATTTTTCAAACATCGCGCGttt < 1:7363/76‑1 (MQ=255) | TATCCAGCTCCTGATACAGCACCTGAGCCACTTTCATGCTGTTCACTTCGTTAAACCCCTGTCGCAAAATGCCTTCCGTCGAGGCTGCCACTTTCAGCGCAGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTT > REL606/2165109‑2165253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |