Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	152.3 / NA	46	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/22);  total (24/22)

CCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193716‑2193851                                                                |                                                                         ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              >  2:1067435/1‑76 (MQ=255)   ggCGTCCAGGCGCGTCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  2:990954/76‑1 (MQ=255)   ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:881913/76‑1 (MQ=255)           ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    >  2:830780/1‑76 (MQ=255)           ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    <  2:596963/76‑1 (MQ=255)              gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGccc                                                 >  2:78204/1‑75 (MQ=255)                ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  1:1145650/76‑1 (MQ=255)                   tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCTTGACGCCTGTAtt                                            <  1:94982/76‑1 (MQ=255)                   tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:533647/1‑76 (MQ=255)                   tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:459339/76‑1 (MQ=255)                    ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:1029958/1‑76 (MQ=255)                    ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  2:508901/76‑1 (MQ=255)                    ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:280045/1‑76 (MQ=255)                    ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  2:1316584/76‑1 (MQ=255)                      tCTGGCTGGCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  2:1313814/76‑1 (MQ=255)                        tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  2:804808/1‑76 (MQ=255)                         ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCNCACCAGATAAAGCATGACGTCTGTATTAAACAg                                      <  2:1215114/76‑1 (MQ=255)                         ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:7779/76‑1 (MQ=255)                          gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  2:1024040/1‑76 (MQ=255)                          gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  1:859246/1‑76 (MQ=255)                             gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:611749/76‑1 (MQ=255)                             gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:347435/1‑76 (MQ=255)                               cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:1029958/76‑1 (MQ=255)                                 gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:6547/76‑1 (MQ=255)                                 gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:306647/76‑1 (MQ=255)                                 gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:160252/76‑1 (MQ=255)                                  aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:130034/1‑76 (MQ=255)                                  aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:513125/1‑76 (MQ=255)                                   aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  1:378201/76‑1 (MQ=255)                                   aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  2:676449/1‑76 (MQ=255)                                   aaTCAGCCCCAATAAAAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  2:730104/1‑76 (MQ=255)                                          cccAATAACAGCAAAACGCCAATCCCCACCAGNTAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATNgcg                     >  2:1331895/1‑76 (MQ=255)                                             aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:823878/76‑1 (MQ=255)                                                aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:596479/1‑76 (MQ=255)                                                  cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:129085/76‑1 (MQ=255)                                                  cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:1157325/76‑1 (MQ=255)                                                  cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  1:976826/1‑76 (MQ=255)                                                      aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:143541/76‑1 (MQ=255)                                                      aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:635582/76‑1 (MQ=255)                                                      aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:874449/1‑76 (MQ=255)                                                        aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTNTTTACCGATGGCGCATCGCGCACCAgc       <  1:1420088/76‑1 (MQ=255)                                                         aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  2:1102214/1‑76 (MQ=255)                                                          cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     >  1:672189/1‑76 (MQ=255)                                                          cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     >  2:1086278/1‑76 (MQ=255)                                                            ccAATCCCCACCACATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:94125/1‑76 (MQ=255)                                                             cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGGTTGCGCATCGNGCACCAGCCGTca  >  1:182953/1‑76 (MQ=255)                                                             cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1235539/1‑76 (MQ=255)                                                                |                                                                         CCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193716‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑