| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,465,075 | G→A | S65L (TCG→TTG) | ompR ← | osmolarity response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,465,075 | 0 | G | A | 97.9% | 175.0 / NA | 47 | S65L (TCG→TTG) | ompR | osmolarity response regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (27/19); minor base T (1/0); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGA > REL606/3465003‑3465146 | aCTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACa < 1:1150351/76‑1 (MQ=255) cttcttCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATcaacaa > 2:845334/1‑76 (MQ=255) cttcttCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATcaacaa < 1:804126/76‑1 (MQ=255) cttcttCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATcaacaa < 1:622284/76‑1 (MQ=255) ttcttcCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAg > 2:569720/1‑76 (MQ=255) cttcCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGcc > 1:913959/1‑76 (MQ=255) cttcCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGcc > 2:1105900/1‑76 (MQ=255) cccTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCt < 1:814456/76‑1 (MQ=255) cccTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCt < 1:964483/76‑1 (MQ=255) cTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTc > 2:735932/1‑76 (MQ=255) ttgcGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCa < 1:108807/73‑1 (MQ=255) tCGCCGTCNCCATAATGATCGGCATCGGGTTGCTCTGACCACGAAGACGTCGGCAAATCAACAAGCCATCTTCAcc < 1:1249692/76‑1 (MQ=255) tCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCAcc < 1:199975/76‑1 (MQ=255) cGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCTATCTTCACCa < 1:1318105/76‑1 (MQ=255) gCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAg < 1:367810/76‑1 (MQ=255) gTCACCATAATGATCGGCATCGGGTTGCTTTGACTATGAAGACGTCGGCAAATCAACNAGCCATCTTCACCAGGTa < 1:1354247/76‑1 (MQ=255) tCACCATAATGATCGGCATNGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTaa > 1:1335837/1‑76 (MQ=255) ccATAATGATCGGCATCGGGTTGNTCTGANTACGAAGACGTCGGNAATTCATCAAGCCATCTTCACCAGGTAACAt > 2:183831/1‑76 (MQ=255) aTAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTa > 2:154155/1‑76 (MQ=255) aTAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTa < 1:687112/76‑1 (MQ=255) tAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTaa > 2:502895/1‑76 (MQ=255) gATCGGCATCGGGTTGCTCTGACTACGAAGCCGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATcc > 2:271189/1‑76 (MQ=255) gtCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCa < 2:96224/75‑1 (MQ=255) tCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAg < 2:627837/76‑1 (MQ=255) tCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAg > 2:159706/1‑76 (MQ=255) cGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGt < 2:1042022/76‑1 (MQ=255) gCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTAc < 1:987725/76‑1 (MQ=255) gCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTAc < 1:374026/76‑1 (MQ=255) aTCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCa < 1:1191297/76‑1 (MQ=255) tCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCAt > 1:701109/1‑76 (MQ=255) cGGGTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATa > 2:773191/1‑76 (MQ=255) ggTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAg < 2:376404/76‑1 (MQ=255) ggTTGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATCAAATCCAGTACCATAAg > 1:161460/1‑76 (MQ=255) ttGCTCTGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAAACCAGTACCATAAGAt > 2:42888/1‑76 (MQ=255) ctctGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGa > 1:819265/1‑76 (MQ=255) tctGAATACGGAGACATCGGCAAAGCANCAAGCCATCTTCGCCATGTANCATCGNATCGAGTACCATAAGATGGaa < 1:1384182/76‑1 (MQ=255) tGACTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAg > 1:1120879/1‑76 (MQ=255) cTACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGAtt > 2:1086058/1‑76 (MQ=255) tACGNAGACGTCGGCAAATCAACCAGCCATCCTCACCAGNTAACATTAAATCCAGTACCATAAGATGGAAAGATTc < 1:171870/76‑1 (MQ=255) tACGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTc > 1:703688/1‑76 (MQ=255) cGAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCAc > 2:1134388/1‑76 (MQ=255) gAAGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACg > 1:676719/1‑76 (MQ=255) aaGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGa > 2:175196/1‑76 (MQ=255) aaGACGTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGa > 2:870464/1‑76 (MQ=255) gTCGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcag > 2:235185/1‑76 (MQ=255) cGGCAAATCTACAAGCCATCTTCACCAGGAAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcagca > 2:1216156/1‑76 (MQ=255) cGGCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcagca > 2:21788/1‑76 (MQ=255) ggCAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcagcag > 1:1202500/1‑76 (MQ=255) cAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGc > 2:137745/1‑76 (MQ=255) cAAATCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGc > 2:1162142/1‑76 (MQ=255) aaTCAACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGa < 1:1269430/76‑1 (MQ=255) | ACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGA > REL606/3465003‑3465146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |