Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,028,358	G→T	A35E (GCG→GAG)	ECB_02828 ←	Polysialic acid transport protein kpsM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,028,358	0	G	T	100.0%	165.8 / NA	44	A35E (GCG→GAG)	ECB_02828	Polysialic acid transport protein kpsM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/25);  total (19/25)

ATCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTG  >  REL606/3028289‑3028429                                                                      |                                                                        aTCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCcaca                                                                   <  1:1120814/76‑1 (MQ=255) aTCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCcaca                                                                   <  1:1120831/76‑1 (MQ=255)   cGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACaaa                                                                 <  1:869209/76‑1 (MQ=255)    gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat                                                                >  2:293935/1‑76 (MQ=255)    gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat                                                                <  2:346614/76‑1 (MQ=255)    gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat                                                                <  2:441654/76‑1 (MQ=255)    gTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAat                                                                >  1:394200/1‑76 (MQ=255)      gCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAatat                                                              <  1:526554/76‑1 (MQ=255)                 tGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGaa                                                   <  2:583020/76‑1 (MQ=255)                 tGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGaa                                                   >  1:554196/1‑76 (MQ=255)                  gTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAAc                                                  >  2:436430/1‑76 (MQ=255)                  gTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAAc                                                  >  2:768852/1‑76 (MQ=255)                  gTAACCAAAAATGCCCAACAGTAGCAGCAAATGCGCAGAGGGTTCGCGAATCTCCCACAAATATCCCAGACGGAAc                                                  >  1:58001/1‑76 (MQ=255)                   tAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACt                                                 <  2:262283/76‑1 (MQ=255)                        aaaaaTGCCCAACAGTATCAGCAAATGCGCAGAGGGGTCGAGAATCTCCCACAAATANCCCAGACGGAACTTACCa                                            <  1:1105445/76‑1 (MQ=255)                        aaaaaTGCCCAACAGTATCAGCAAAGGCNCAGAGGGTTTCAGAATCTGCCACAAATATCCCAGACGGAACTTACCa                                            <  1:108022/76‑1 (MQ=255)                         aaaaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCaa                                           >  2:277056/1‑76 (MQ=255)                           aaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAg                                         <  1:526805/76‑1 (MQ=255)                              gCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCgt                                      >  1:1125031/1‑76 (MQ=255)                                   aCAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCg                                 <  1:189593/76‑1 (MQ=255)                                      gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt                              <  2:956974/76‑1 (MQ=255)                                      gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt                              <  2:430169/76‑1 (MQ=255)                                      gTATCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAt                              <  2:430158/76‑1 (MQ=255)                                        aTCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTAttt                            <  1:442141/76‑1 (MQ=255)                                         tCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTtc                           >  2:750442/1‑76 (MQ=255)                                         tCAGCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTtc                           >  2:932320/1‑76 (MQ=255)                                            gCAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCg                        >  1:256131/1‑76 (MQ=255)                                             cAAATGCGCGGAGGNTTCGAGAACCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCgn                       <  2:164098/76‑2 (MQ=255)                                             cAAATGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAANGCGTGTTCGTATTTCTCGt                       <  1:1082944/76‑1 (MQ=255)                                              aaaTGCGCAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTa                      <  2:1157160/76‑1 (MQ=255)                                                    gcAGAGGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaata                <  2:440055/76‑1 (MQ=255)                                                      agagGGTTCGAGAATCTCCCACAAATATCCCAGGCGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataat              >  2:1001965/1‑76 (MQ=255)                                                      agagGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataat              <  2:282904/76‑1 (MQ=255)                                                        agGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGc            >  1:952034/1‑76 (MQ=255)                                                        agGGTTCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGc            >  1:937546/1‑76 (MQ=255)                                                            ttCGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCctct        <  2:630165/76‑1 (MQ=255)                                                              cGAGAATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTAc      >  1:588839/1‑76 (MQ=255)                                                               gagaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACg     <  1:819295/76‑1 (MQ=255)                                                               gagaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACg     <  1:436430/76‑1 (MQ=255)                                                                agaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACgg    >  2:187347/1‑76 (MQ=255)                                                                agaATCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACgg    >  1:249216/1‑76 (MQ=255)                                                                  aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg  >  2:198652/1‑76 (MQ=255)                                                                  aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg  >  2:51533/1‑76 (MQ=255)                                                                  aaTCTCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTg  <  1:614079/76‑1 (MQ=255)                                                                      |                                                                        ATCGTGCGGTGCATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTG  >  REL606/3028289‑3028429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑