| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,243 | A→G | intergenic (‑169/+433) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,243 | 0 | A | G | 94.4% | 130.4 / ‑0.9 | 36 | intergenic (‑169/+433) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (14/20); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATA > REL606/1004170‑1004312 | tgtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataagga < 2:355400/76‑1 (MQ=255)tgtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataagga < 1:398813/76‑1 (MQ=255) cTTTATGTGTCTGNAGGCATCTTTCCATTAAAACTAACGTTTCGCGACCGTGAAAGTGCTACAAAGATAAGGAttt < 2:1064251/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt > 1:523642/1‑76 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTg < 1:182843/76‑1 (MQ=255) aTGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGAGCTACAAAGATAAGGTTTTGGtt > 2:836484/1‑76 (MQ=255) tgtgTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGttt < 2:587906/76‑1 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc > 1:785713/1‑76 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 1:274457/76‑1 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACATTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 1:274465/76‑1 (MQ=255) tgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCa > 1:627720/1‑76 (MQ=255) gtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaa < 1:444803/76‑1 (MQ=255) gtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaa > 1:934644/1‑76 (MQ=255) gCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAg < 1:846035/76‑1 (MQ=255) gCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAg < 1:626109/76‑1 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa > 2:119405/1‑76 (MQ=255) gtCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGTTTTGGTTTCAAAAAGAAAAat > 1:314286/2‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat > 1:314275/1‑76 (MQ=255) tCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAata < 2:441762/76‑1 (MQ=255) aTTAAATCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGTATTTGGTTTCAAAAAGAAAAATATGTAAcc < 1:1064146/76‑1 (MQ=255) aTTAAATCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGTATTTGGTTTCAAAAAGAAAAATATGTAAcc < 1:1064147/76‑1 (MQ=255) aTTAAAGCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAAcc < 2:1134691/76‑1 (MQ=255) aTTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAAcc > 2:261407/1‑76 (MQ=255) ttAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCa < 2:803064/76‑1 (MQ=255) tAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaa < 1:413452/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa > 2:328970/1‑76 (MQ=255) tAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGAATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaa > 2:431605/1‑76 (MQ=255) aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaaa < 1:318476/76‑1 (MQ=255) cGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAt > 1:504801/1‑76 (MQ=255) cTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGa < 1:167903/76‑1 (MQ=255) aCCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAAc < 1:201233/76‑1 (MQ=255) ccGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTGACCAAAAGTANAATTTAAGGagcg > 1:149845/1‑73 (MQ=255) ccGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt < 2:475342/76‑1 (MQ=255) cGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACtt < 1:1120433/76‑1 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca < 1:426092/76‑1 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca > 2:751467/1‑76 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca > 2:751480/1‑76 (MQ=255) tACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg > 1:1135245/1‑76 (MQ=255) cAAAGATAAGGAATTGGTTTCAAAAAGAAAAANATCTAACCNAAAGTCAAATTTAAGGAACNTTGTGAACACCGTc > 2:968305/1‑76 (MQ=255) aaagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa > 1:486731/1‑76 (MQ=255) aaagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa > 1:486720/1‑76 (MQ=255) agataaggatTTGGTTTCAACAGGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata > 1:5521/1‑76 (MQ=255) | TGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATA > REL606/1004170‑1004312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |