Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,415,090	T→C	H30R (CAC→CGC)	ydaE ←	conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,415,090	0	T	C	96.8%	119.7 / ‑3.4	31	H30R (CAC→CGC)	ydaE	conserved protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (8/22);  total (8/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

ATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACAT  >  REL606/1415017‑1415161                                                                          |                                                                        aTGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATANTCNCGGTTCGTCTTTTGNTAAAATCCCgcgc                                                                       >  2:1049897/1‑76 (MQ=255)   gtgCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCAt                                                                     <  2:1001/76‑1 (MQ=255)            ggTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCACAtgaagtgaag                                                            >  1:796647/1‑76 (MQ=255)                    tCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCttt                                                    <  2:413684/76‑1 (MQ=255)                    tCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCttt                                                    <  2:413669/76‑1 (MQ=255)                     cGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTc                                                   >  2:917277/1‑76 (MQ=255)                     cGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTc                                                   <  2:1078970/76‑1 (MQ=255)                      ggCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCa                                                  <  1:1008919/76‑1 (MQ=255)                      ggCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCa                                                  <  1:727029/76‑1 (MQ=255)                       gCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCaa                                                 <  2:1158062/76‑1 (MQ=255)                         acaAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATa                                               <  2:279892/76‑1 (MQ=255)                         acaAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATa                                               <  1:424832/76‑1 (MQ=255)                            aGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgct                                            >  2:615437/1‑76 (MQ=255)                               ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttg                                         >  1:44246/1‑76 (MQ=255)                                  aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttgctt                                      >  2:378996/1‑76 (MQ=255)                                   cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttgcttt                                     <  2:294574/76‑1 (MQ=255)                                    tGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCCTTCTTTCAATAGCTTTGCTttc                                    >  2:44149/1‑76 (MQ=255)                                      cAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTttctt                                  <  2:711361/76‑1 (MQ=255)                                       aaTATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTttcttc                                 <  2:1032208/76‑1 (MQ=255)                                        atatTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCa                                <  2:673911/76‑1 (MQ=255)                                         tatTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCaa                               <  1:199941/76‑1 (MQ=255)                                           ttttCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACg                             <  2:606653/76‑1 (MQ=255)                                           ttttCTCGGTTCGTCTTTTGATAAAAGCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACg                             <  2:606639/76‑1 (MQ=255)                                                         cTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAg               >  1:276842/1‑76 (MQ=255)                                                         cTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTNTTTGCAAAg               <  1:24289/76‑1 (MQ=255)                                                          ttttGATATAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTGTTGCAAAgg              >  1:42131/1‑76 (MQ=255)                                                           tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt             <  2:651947/76‑1 (MQ=255)                                                           tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt             <  2:651956/76‑1 (MQ=255)                                                           tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt             <  2:958067/76‑1 (MQ=255)                                                               aTAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTa         <  2:605900/76‑1 (MQ=255)                                                                     tCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGcaca   <  1:213354/76‑1 (MQ=255)                                                                      cccGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACat  <  2:476002/76‑1 (MQ=255)                                                                          |                                                                        ATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACAT  >  REL606/1415017‑1415161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑